Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1416823..1417466 Replicon chromosome
Accession NZ_CP098141
Organism Klebsiella pneumoniae strain Z0117KP0025

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag NBY50_RS08430 Protein ID WP_032433387.1
Coordinates 1417050..1417466 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag NBY50_RS08425 Protein ID WP_001261276.1
Coordinates 1416823..1417053 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY50_RS08410 (1411845) 1411845..1412932 + 1088 Protein_1364 transcriptional repressor PifC -
NBY50_RS08415 (1412935) 1412935..1415175 + 2241 WP_045326788.1 P-loop NTPase fold protein -
NBY50_RS08420 (1415703) 1415703..1416518 - 816 WP_032433388.1 hypothetical protein -
NBY50_RS08425 (1416823) 1416823..1417053 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NBY50_RS08430 (1417050) 1417050..1417466 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
NBY50_RS08435 (1417622) 1417622..1418602 + 981 WP_032433385.1 hypothetical protein -
NBY50_RS08440 (1418797) 1418797..1420368 - 1572 WP_032433383.1 AAA family ATPase -
NBY50_RS08445 (1420687) 1420687..1420935 + 249 WP_032433382.1 hypothetical protein -
NBY50_RS08450 (1420994) 1420994..1421512 + 519 WP_045326794.1 hypothetical protein -
NBY50_RS08455 (1421543) 1421543..1422034 + 492 WP_032433378.1 hypothetical protein -
NBY50_RS08460 (1422094) 1422094..1422297 + 204 WP_032433376.1 HHA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1401459..1445193 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T246680 WP_032433387.1 NZ_CP098141:1417050-1417466 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T246680 NZ_CP098141:1417050-1417466 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT246680 WP_001261276.1 NZ_CP098141:1416823-1417053 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT246680 NZ_CP098141:1416823-1417053 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References