Detailed information of TA system TA024663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 318630..319170
• Replicon: chromosome
• Accession: NC_013960
• Organism: Nitrosococcus halophilus Nc 4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5BUW6
• Locus tag: NHAL_RS01570
• Protein ID: WP_013031412.1
• Coordinates: 318630..318950 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D5BUW7
• Locus tag: NHAL_RS01575
• Protein ID: WP_013031413.1
• Coordinates: 318937..319170 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAL_RS20080	313825..314010	+	186	WP_203434348.1	DUF1365 family protein	-
NHAL_RS01540	314007..315311	+	1305	WP_013031406.1	cyclopropane-fatty-acyl-phospholipid synthase family protein	-
NHAL_RS01545	315691..316722	-	1032	WP_013031407.1	hypothetical protein	-
NHAL_RS01550	316793..317038	-	246	Protein_307	transposase	-
NHAL_RS01555	317076..317480	-	405	WP_013031409.1	hypothetical protein	-
NHAL_RS01560	317914..318201	-	288	WP_013031410.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
NHAL_RS01565	318198..318443	-	246	WP_203434349.1	CopG family ribbon-helix-helix protein	-
NHAL_RS01570	318630..318950	-	321	WP_013031412.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NHAL_RS01575	318937..319170	-	234	WP_013031413.1	hypothetical protein	Antitoxin
NHAL_RS01580	319233..320387	-	1155	WP_013031414.1	MFS transporter	-
NHAL_RS01585	320706..321641	+	936	WP_013031416.1	malate dehydrogenase	-
NHAL_RS01590	321868..322659	+	792	WP_013031417.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11783.94 Da
• Isoelectric Point: 10.9006

>T24663 WP_013031412.1 NC_013960:c318950-318630 [Nitrosococcus halophilus Nc 4]
MNRGEVYWVNLDPTQGSEIRKQRPCVLVGATPINKARRTVIVVPLSTSAKPNPPLTIPIQCLNRQIVAVCDQIRAIDKTR
LLKSAGRLSKEDMKAINEGLRQVLVL

Nucleotide

• Length: 321 bp

>T24663 NC_013960:c318950-318630 [Nitrosococcus halophilus Nc 4]
ATGAATCGTGGTGAGGTCTATTGGGTTAATCTTGACCCTACCCAGGGATCAGAGATCCGCAAGCAACGTCCTTGTGTGCT
AGTAGGCGCCACCCCCATTAATAAAGCTCGGCGCACAGTCATAGTCGTACCCCTTTCCACCTCCGCCAAGCCCAACCCCC
CACTCACCATCCCCATACAGTGCCTAAACCGCCAAATTGTAGCAGTCTGCGACCAAATTCGCGCCATAGATAAGACTCGA
CTGCTAAAGTCCGCAGGCCGCTTATCAAAAGAAGACATGAAGGCCATCAATGAGGGATTAAGACAAGTTCTAGTACTTTA
A

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8929.18 Da
• Isoelectric Point: 4.4012

>AT24663 WP_013031413.1 NC_013960:c319170-318937 [Nitrosococcus halophilus Nc 4]
MQKVLFSLPDDLVTRMRASIPSKQRSKVIACLLEQEIQKREQALYECALKVEADETLKEEMKDWEATQGDGIENESW

Nucleotide

• Length: 234 bp

>AT24663 NC_013960:c319170-318937 [Nitrosococcus halophilus Nc 4]
ATGCAAAAAGTTTTATTTTCCCTACCTGATGATTTAGTCACTCGAATGAGAGCTAGCATCCCTTCTAAACAGCGAAGCAA
GGTCATCGCTTGCTTGCTGGAGCAGGAAATTCAAAAGCGGGAACAAGCCCTTTATGAGTGCGCCTTGAAAGTCGAAGCAG
ATGAGACCCTAAAAGAAGAAATGAAGGACTGGGAAGCCACCCAGGGCGATGGAATAGAAAATGAATCGTGGTGA

Structures

Toxin

• AlphaFold DB: D5BUW6

Antitoxin

• AlphaFold DB: D5BUW7