Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1919402..1920233 Replicon chromosome
Accession NZ_CP097724
Organism Escherichia coli strain MS1494

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag M9O75_RS09135 Protein ID WP_000854815.1
Coordinates 1919402..1919776 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2T3TJJ3
Locus tag M9O75_RS09140 Protein ID WP_001285590.1
Coordinates 1919865..1920233 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O75_RS09095 (1914797) 1914797..1915963 + 1167 WP_000830157.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
M9O75_RS09100 (1916082) 1916082..1916555 + 474 WP_001105395.1 DNA gyrase inhibitor SbmC -
M9O75_RS09105 (1916754) 1916754..1917812 + 1059 WP_001200890.1 FUSC family protein -
M9O75_RS09110 (1917984) 1917984..1918313 + 330 WP_000450409.1 DUF496 family protein -
M9O75_RS09115 (1918414) 1918414..1918548 - 135 WP_059338447.1 EutP/PduV family microcompartment system protein -
M9O75_RS09120 (1918650) 1918650..1918796 + 147 Protein_1785 transposase domain-containing protein -
M9O75_RS09125 (1919085) 1919085..1919165 - 81 Protein_1786 hypothetical protein -
M9O75_RS09130 (1919211) 1919211..1919405 - 195 WP_000988600.1 DUF5983 family protein -
M9O75_RS09135 (1919402) 1919402..1919776 - 375 WP_000854815.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
M9O75_RS09140 (1919865) 1919865..1920233 - 369 WP_001285590.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
M9O75_RS09145 (1920307) 1920307..1920528 - 222 WP_000692300.1 DUF987 domain-containing protein -
M9O75_RS09150 (1920597) 1920597..1920893 - 297 Protein_1791 hypothetical protein -
M9O75_RS09155 (1921320) 1921320..1921520 - 201 WP_001297900.1 hypothetical protein -
M9O75_RS09160 (1921719) 1921719..1922288 + 570 WP_021576243.1 inovirus Gp2 family protein -
M9O75_RS09165 (1922548) 1922548..1922949 + 402 WP_122989205.1 putative zinc ribbon protein -
M9O75_RS09170 (1922937) 1922937..1923345 + 409 Protein_1795 zinc-ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T246180 WP_000854815.1 NZ_CP097724:c1919776-1919402 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T246180 NZ_CP097724:c1919776-1919402 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTTCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATCGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13608.50 Da        Isoelectric Point: 6.6242

>AT246180 WP_001285590.1 NZ_CP097724:c1920233-1919865 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLYSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT246180 NZ_CP097724:c1920233-1919865 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
ACAGCAATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A2T3TJJ3

References