Detailed information of TA system TA245688

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94730..95156
• Replicon: plasmid pKPC2_140253
• Accession: NZ_CP097627
• Organism: Klebsiella pneumoniae strain 140253

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: M9P58_RS27565
• Protein ID: WP_001372321.1
• Coordinates: 94730..94855 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	94932..95156 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M9P58_RS27530 (89785)	89785..90012	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
M9P58_RS27535 (90106)	90106..90792	-	687	WP_015059009.1	PAS domain-containing protein	-
M9P58_RS27540 (90983)	90983..91366	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
M9P58_RS27545 (91643)	91643..92290	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
M9P58_RS27550 (92587)	92587..93408	-	822	WP_001234445.1	DUF932 domain-containing protein	-
M9P58_RS27555 (93519)	93519..93815	-	297	WP_001272251.1	hypothetical protein	-
M9P58_RS27560 (94115)	94115..94411	+	297	Protein_121	hypothetical protein	-
M9P58_RS27565 (94730)	94730..94855	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
M9P58_RS27570 (94797)	94797..94946	-	150	Protein_123	plasmid maintenance protein Mok	-
- (94932)	94932..95156	-	225	NuclAT_0	-	Antitoxin
- (94932)	94932..95156	-	225	NuclAT_0	-	Antitoxin
- (94932)	94932..95156	-	225	NuclAT_0	-	Antitoxin
- (94932)	94932..95156	-	225	NuclAT_0	-	Antitoxin
M9P58_RS27575 (95168)	95168..95887	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
M9P58_RS27580 (95884)	95884..96318	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
M9P58_RS27585 (96387)	96387..98410	-	2024	Protein_126	ParB/RepB/Spo0J family partition protein	-
M9P58_RS27590 (98471)	98471..98704	-	234	WP_000006003.1	DUF905 family protein	-
M9P58_RS27595 (98762)	98762..99289	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
M9P58_RS27600 (99591)	99591..100052	+	462	Protein_129	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2	-	1..155793	155793

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T245688 WP_001372321.1 NZ_CP097627:c94855-94730 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT245688 NZ_CP097627:c95156-94932 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin