Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1449066..1449856 Replicon chromosome
Accession NZ_CP097529
Organism Pseudomonas putida strain KT2440 isolate pSEVA631

Toxin (Protein)


Gene name yhaV Uniprot ID Q88NE5
Locus tag M8Z99_RS06615 Protein ID WP_010952397.1
Coordinates 1449066..1449533 (-) Length 156 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID I7C311
Locus tag M8Z99_RS06620 Protein ID WP_014859917.1
Coordinates 1449530..1449856 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Z99_RS06595 (M8Z99_06580) 1444229..1445758 + 1530 WP_010952395.1 efflux transporter outer membrane subunit -
M8Z99_RS06600 (M8Z99_06585) 1445755..1447932 + 2178 WP_003251716.1 FUSC family protein -
M8Z99_RS06605 (M8Z99_06590) 1447922..1448122 + 201 WP_003251718.1 DUF1656 domain-containing protein -
M8Z99_RS06610 (M8Z99_06595) 1448133..1448993 + 861 WP_010952396.1 HlyD family secretion protein -
M8Z99_RS06615 (M8Z99_06600) 1449066..1449533 - 468 WP_010952397.1 type II toxin-antitoxin system YhaV family toxin Toxin
M8Z99_RS06620 (M8Z99_06605) 1449530..1449856 - 327 WP_014859917.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
M8Z99_RS06625 (M8Z99_06610) 1449949..1450395 - 447 WP_010952399.1 universal stress protein -
M8Z99_RS06630 (M8Z99_06615) 1450669..1451574 + 906 WP_010952400.1 LysR family transcriptional regulator -
M8Z99_RS06635 (M8Z99_06620) 1451691..1453232 + 1542 WP_010952401.1 MFS transporter -
M8Z99_RS06640 (M8Z99_06625) 1453261..1454322 + 1062 WP_049587435.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(17-152)

Antitoxin

PrlF antitoxin

(8-106)


Sequences

Toxin        


Download         Length: 156 a.a.        Molecular weight: 17962.34 Da        Isoelectric Point: 9.7963

>T245541 WP_010952397.1 NZ_CP097529:c1449533-1449066 [Pseudomonas putida]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTKRNTFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWINDESSKRAYESSDDAYKVFQKMLHSGNPPDDWDQLLQEAAAD

Download         Length: 468 bp

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11919.40 Da        Isoelectric Point: 4.3722

>AT245541 WP_014859917.1 NZ_CP097529:c1449856-1449530 [Pseudomonas putida]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Download         Length: 327 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88NE5

Antitoxin

Source ID Structure
AlphaFold DB I7C311

References