Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58330..59066 Replicon plasmid pKPTCM-1
Accession NZ_CP097386
Organism Klebsiella pneumoniae strain KPTCM

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag M6G79_RS25170 Protein ID WP_004098919.1
Coordinates 58584..59066 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag M6G79_RS25165 Protein ID WP_004213599.1
Coordinates 58330..58596 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M6G79_RS25145 (M6G79_25120) 55843..56847 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
M6G79_RS25150 (M6G79_25125) 57133..57627 + 495 WP_004213594.1 hypothetical protein -
M6G79_RS25155 (M6G79_25130) 57688..57891 + 204 WP_004213596.1 HHA domain-containing protein -
M6G79_RS25160 (M6G79_25135) 57905..58135 + 231 WP_004213598.1 hypothetical protein -
M6G79_RS25165 (M6G79_25140) 58330..58596 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
M6G79_RS25170 (M6G79_25145) 58584..59066 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
M6G79_RS25175 (M6G79_25150) 59267..60670 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
M6G79_RS25180 (M6G79_25155) 60699..61331 - 633 WP_001567369.1 hypothetical protein -
M6G79_RS25185 (M6G79_25160) 61770..62997 + 1228 Protein_61 IS3 family transposase -
M6G79_RS25190 (M6G79_25165) 62993..63388 - 396 Protein_62 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iucA / iucB / iucC / iucD / iutA / rmpA 1..167179 167179
- inside IScluster/Tn - - 47012..62997 15985


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T245370 WP_004098919.1 NZ_CP097386:58584-59066 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT245370 WP_004213599.1 NZ_CP097386:58330-58596 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References