Detailed information of TA system TA245328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3695380..3697069
• Replicon: chromosome
• Accession: NZ_CP097370
• Organism: Escherichia coli strain AGR5151

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: M7748_RS17815
• Protein ID: WP_000365807.1
• Coordinates: 3695380..3696300 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: M7748_RS17820
• Protein ID: WP_001295795.1
• Coordinates: 3696287..3697069 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M7748_RS17775 (3690853)	3690853..3691563	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
M7748_RS17780 (3691870)	3691870..3692199	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
M7748_RS17785 (3692211)	3692211..3692377	-	167	Protein_3491	tail fiber assembly protein	-
M7748_RS17790 (3692448)	3692448..3693062	-	615	WP_001019923.1	YagU family protein	-
M7748_RS17795 (3693277)	3693277..3693462	-	186	WP_000146240.1	hypothetical protein	-
M7748_RS17800 (3693511)	3693511..3693669	+	159	Protein_3494	integrase	-
M7748_RS17805 (3693896)	3693896..3694231	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
M7748_RS17810 (3694439)	3694439..3695215	+	777	WP_000171155.1	site-specific integrase	-
M7748_RS17815 (3695380)	3695380..3696300	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M7748_RS17820 (3696287)	3696287..3697069	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M7748_RS17825 (3697353)	3697353..3700451	+	3099	WP_000673070.1	type I restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3656261..3704132	47871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T245328 WP_000365807.1 NZ_CP097370:c3696300-3695380 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT245328 WP_001295795.1 NZ_CP097370:c3697069-3696287 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0