Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3619042..3619660 Replicon chromosome
Accession NZ_CP097360
Organism Escherichia coli strain AGR4587

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag M7749_RS17720 Protein ID WP_001291435.1
Coordinates 3619442..3619660 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag M7749_RS17715 Protein ID WP_000344800.1
Coordinates 3619042..3619416 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M7749_RS17705 (3614131) 3614131..3615324 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M7749_RS17710 (3615347) 3615347..3618496 + 3150 WP_001132478.1 efflux RND transporter permease AcrB -
M7749_RS17715 (3619042) 3619042..3619416 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
M7749_RS17720 (3619442) 3619442..3619660 + 219 WP_001291435.1 HHA domain-containing protein Toxin
M7749_RS17725 (3619833) 3619833..3620384 + 552 WP_000102539.1 maltose O-acetyltransferase -
M7749_RS17730 (3620500) 3620500..3620970 + 471 WP_000136192.1 YlaC family protein -
M7749_RS17735 (3621134) 3621134..3622684 + 1551 WP_001545832.1 cyclic-guanylate-specific phosphodiesterase PdeB -
M7749_RS17740 (3622726) 3622726..3623079 - 354 WP_000878147.1 DUF1428 family protein -
M7749_RS17750 (3623458) 3623458..3623769 + 312 WP_000409908.1 MGMT family protein -
M7749_RS17755 (3623800) 3623800..3624372 - 573 WP_250011652.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T245280 WP_001291435.1 NZ_CP097360:3619442-3619660 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T245280 NZ_CP097360:3619442-3619660 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT245280 WP_000344800.1 NZ_CP097360:3619042-3619416 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT245280 NZ_CP097360:3619042-3619416 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References