Detailed information of TA system TA024528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 6683632..6684263
• Replicon: chromosome
• Accession: NC_013729
• Organism: Kribbella flavida DSM 17836

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D2PVK8
• Locus tag: KFLA_RS31025
• Protein ID: WP_012923801.1
• Coordinates: 6683850..6684263 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D2PVK7
• Locus tag: KFLA_RS31020
• Protein ID: WP_012923800.1
• Coordinates: 6683632..6683853 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFLA_RS30995	6678749..6679816	+	1068	WP_148256780.1	peptidoglycan-binding protein	-
KFLA_RS31000	6679813..6680475	+	663	WP_012923796.1	ABC transporter ATP-binding protein	-
KFLA_RS31005	6680475..6681689	+	1215	WP_012923797.1	ABC transporter permease	-
KFLA_RS31010	6681710..6682660	-	951	WP_012923798.1	NADH-quinone oxidoreductase subunit H	-
KFLA_RS31015	6682735..6683604	+	870	WP_202797045.1	NAD(P)H-binding protein	-
KFLA_RS31020	6683632..6683853	+	222	WP_012923800.1	antitoxin	Antitoxin
KFLA_RS31025	6683850..6684263	+	414	WP_012923801.1	PIN domain nuclease	Toxin
KFLA_RS31030	6684254..6685042	-	789	WP_012923802.1	NADH-quinone oxidoreductase subunit C	-
KFLA_RS31035	6685039..6685602	-	564	WP_041289557.1	NADH-quinone oxidoreductase subunit B	-
KFLA_RS31040	6685858..6687339	+	1482	WP_041290680.1	cytosine permease	-
KFLA_RS31045	6687341..6687883	+	543	WP_012923805.1	cysteine hydrolase	-
KFLA_RS31050	6687904..6688611	+	708	WP_148257142.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14737.86 Da
• Isoelectric Point: 4.6602

>T24528 WP_012923801.1 NC_013729:6683850-6684263 [Kribbella flavida DSM 17836]
VTETTWLIDKSALVRLGDGPQIDQWANRIDRGLVHITPVTLLEVGFSTRSAEEHRMTLQFPPLSSMPVVNSTPAIESRAV
AVQALLAATGQHRAPSVPDLLIAATAELAGLTVLHLDEDFELIAGITGQPVERLSQV

Nucleotide

• Length: 414 bp

>T24528 NC_013729:6683850-6684263 [Kribbella flavida DSM 17836]
GTGACCGAGACGACCTGGCTGATCGACAAGTCCGCGCTCGTTCGGCTCGGCGACGGGCCACAGATCGACCAGTGGGCGAA
TCGGATCGATCGCGGCCTGGTGCACATCACGCCGGTCACGTTGCTCGAGGTCGGCTTCTCGACCCGGTCGGCCGAGGAGC
ACCGGATGACGCTGCAGTTCCCGCCCCTCTCGTCGATGCCGGTGGTCAACTCGACCCCGGCGATCGAGAGCCGGGCAGTG
GCCGTGCAGGCGTTGCTGGCCGCGACCGGGCAACATCGCGCTCCGTCGGTGCCGGACCTGCTGATCGCGGCGACCGCTGA
GCTGGCCGGGCTGACCGTCCTGCACCTCGACGAGGACTTCGAGCTGATCGCCGGTATCACCGGGCAACCGGTCGAACGTC
TCTCTCAGGTCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8366.40 Da
• Isoelectric Point: 6.7241

>AT24528 WP_012923800.1 NC_013729:6683632-6683853 [Kribbella flavida DSM 17836]
MSDVLIRDVPDDVLAVLDSRARHLGLSRNEYLRRRLSQEAKRSGETVTAAHLRRLSHAIRDLDDPDVMDQAWS

Nucleotide

• Length: 222 bp

>AT24528 NC_013729:6683632-6683853 [Kribbella flavida DSM 17836]
ATGAGCGATGTGCTGATCCGCGACGTGCCGGACGACGTACTGGCGGTGCTCGACAGCCGCGCGCGTCACCTCGGTCTGTC
CAGGAACGAGTACCTTCGACGCCGCCTCAGCCAGGAGGCGAAGAGGTCCGGTGAGACCGTGACGGCCGCGCATCTGCGGC
GGTTGTCGCACGCGATCCGGGATCTCGACGATCCCGACGTGATGGACCAGGCCTGGTCGTGA

Structures

Toxin

• AlphaFold DB: D2PVK8

Antitoxin

• AlphaFold DB: D2PVK7