Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 212526..213168 Replicon chromosome
Accession NZ_CP097257
Organism Bacillus thuringiensis strain Bt Gxmzu777-1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag M3Y14_RS01195 Protein ID WP_000635965.1
Coordinates 212818..213168 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag M3Y14_RS01190 Protein ID WP_000004570.1
Coordinates 212526..212813 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3Y14_RS01165 (M3Y14_01160) 207730..208692 + 963 WP_264539971.1 UV DNA damage repair endonuclease UvsE -
M3Y14_RS01170 (M3Y14_01165) 208820..209368 - 549 WP_219916894.1 rhomboid family intramembrane serine protease -
M3Y14_RS01175 (M3Y14_01170) 209461..209820 + 360 WP_002029438.1 holo-ACP synthase -
M3Y14_RS01180 (M3Y14_01175) 209977..210927 + 951 WP_219921113.1 outer membrane lipoprotein carrier protein LolA -
M3Y14_RS01185 (M3Y14_01180) 211045..212214 + 1170 WP_002151404.1 alanine racemase -
M3Y14_RS01190 (M3Y14_01185) 212526..212813 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
M3Y14_RS01195 (M3Y14_01190) 212818..213168 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M3Y14_RS01200 (M3Y14_01195) 213237..215405 + 2169 WP_264541140.1 Tex family protein -
M3Y14_RS01205 (M3Y14_01200) 215464..215580 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
M3Y14_RS01210 (M3Y14_01205) 215790..216245 + 456 WP_264539972.1 SprT family protein -
M3Y14_RS01270 (M3Y14_01265) 217644..218117 + 474 WP_219916896.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T245065 WP_000635965.1 NZ_CP097257:212818-213168 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT245065 WP_000004570.1 NZ_CP097257:212526-212813 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References