Detailed information of TA system TA024485

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2025965..2026796
• Replicon: chromosome
• Accession: NC_013654
• Organism: Escherichia coli SE15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: ECSF_RS10140
• Protein ID: WP_000854814.1
• Coordinates: 2026422..2026796 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: C0ST46
• Locus tag: ECSF_RS10135
• Protein ID: WP_001285587.1
• Coordinates: 2025965..2026333 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSF_RS10100	2021022..2022092	+	1071	WP_000102631.1	patatin-like phospholipase family protein	-
ECSF_RS10105	2022228..2022905	+	678	WP_001362823.1	hypothetical protein	-
ECSF_RS10110	2022921..2023331	+	411	WP_000846704.1	hypothetical protein	-
ECSF_RS10115	2023552..2024373	+	822	WP_001234710.1	DUF945 domain-containing protein	-
ECSF_RS10120	2024636..2025109	+	474	WP_001385393.1	antirestriction protein	-
ECSF_RS10125	2025125..2025601	+	477	WP_001186773.1	RadC family protein	-
ECSF_RS10130	2025664..2025885	+	222	WP_000692323.1	DUF987 domain-containing protein	-
ECSF_RS10135	2025965..2026333	+	369	WP_001285587.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
ECSF_RS10140	2026422..2026796	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
ECSF_RS10145	2026793..2026987	+	195	WP_000988601.1	hypothetical protein	-
ECSF_RS26200	2027000..2027113	+	114	WP_001161660.1	DUF957 domain-containing protein	-
ECSF_RS10155	2027476..2028222	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
ECSF_RS10160	2028237..2029778	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
ECSF_RS10165	2030022..2030168	-	147	Protein_1930	transposase domain-containing protein	-
ECSF_RS10170	2030222..2030404	+	183	WP_001016348.1	hypothetical protein	-
ECSF_RS10175	2030505..2030834	-	330	WP_000450409.1	DUF496 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T24485 WP_000854814.1 NC_013654:2026422-2026796 [Escherichia coli SE15]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T24485 NC_013654:2026422-2026796 [Escherichia coli SE15]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13577.44 Da
• Isoelectric Point: 6.3139

>AT24485 WP_001285587.1 NC_013654:2025965-2026333 [Escherichia coli SE15]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT24485 NC_013654:2025965-2026333 [Escherichia coli SE15]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50