Detailed information of TA system TA243890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2828509..2829146
• Replicon: chromosome
• Accession: NZ_CP096845
• Organism: Mycobacterium tuberculosis variant bovis strain Mb1101

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: M1777_RS13335
• Protein ID: WP_003413180.1
• Coordinates: 2828509..2828904 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: M1777_RS13340
• Protein ID: WP_003413183.1
• Coordinates: 2828901..2829146 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1777_RS13295 (M1777_13305)	2824594..2825253	+	660	WP_003900846.1	LppA family lipoprotein	-
M1777_RS13300 (M1777_13310)	2825250..2825909	+	660	WP_003900847.1	LppA family lipoprotein	-
M1777_RS13305 (M1777_13315)	2825906..2826568	+	663	WP_003900848.1	LppA family lipoprotein	-
M1777_RS13310 (M1777_13320)	2826565..2826843	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
M1777_RS13315 (M1777_13325)	2826936..2827349	+	414	WP_003413164.1	PIN domain nuclease	-
M1777_RS13320 (M1777_13330)	2827388..2827645	+	258	WP_003413167.1	CopG family transcriptional regulator	-
M1777_RS13325 (M1777_13335)	2827642..2828019	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
M1777_RS13330 (M1777_13340)	2828035..2828409	-	375	WP_003413177.1	hypothetical protein	-
M1777_RS13335 (M1777_13345)	2828509..2828904	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
M1777_RS13340 (M1777_13350)	2828901..2829146	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
M1777_RS13345 (M1777_13355)	2829557..2829976	-	420	WP_273585834.1	A24 family peptidase	-
M1777_RS13350 (M1777_13360)	2829988..2830796	-	809	Protein_2635	shikimate dehydrogenase	-
M1777_RS13355 (M1777_13365)	2830793..2832046	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
M1777_RS13360 (M1777_13370)	2832039..2832551	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T243890 WP_003413180.1 NZ_CP096845:c2828904-2828509 [Mycobacterium tuberculosis variant bovis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T243890 NZ_OY019088:c832230-831979 [Escherichia coli O25b:H4-ST131]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT243890 WP_003413183.1 NZ_CP096845:c2829146-2828901 [Mycobacterium tuberculosis variant bovis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT243890 NZ_OY019088:c831982-831728 [Escherichia coli O25b:H4-ST131]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31