Detailed information of TA system TA243852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 694899..695545
• Replicon: chromosome
• Accession: NZ_CP096845
• Organism: Mycobacterium tuberculosis variant bovis strain Mb1101

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: M1777_RS03150
• Protein ID: WP_003403137.1
• Coordinates: 694899..695312 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: M1777_RS03155
• Protein ID: WP_003403139.1
• Coordinates: 695309..695545 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1777_RS03130 (M1777_03130)	690982..692532	+	1551	WP_003403119.1	MlaD family protein	-
M1777_RS03135 (M1777_03135)	692584..692976	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
M1777_RS03140 (M1777_03140)	692973..693230	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
M1777_RS03145 (M1777_03145)	693413..694648	-	1236	WP_003403128.1	ATP-binding protein	-
M1777_RS03150 (M1777_03150)	694899..695312	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1777_RS03155 (M1777_03155)	695309..695545	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
M1777_RS03160 (M1777_03160)	695649..696155	-	507	WP_003900973.1	two component system sensor kinase HK1	-
M1777_RS03165 (M1777_03165)	696269..696799	-	531	WP_003917320.1	two component system sensor kinase HK2	-
M1777_RS03170 (M1777_03170)	696783..697478	-	696	WP_197044064.1	two-component system response regulator TcrA	-
M1777_RS03175 (M1777_03175)	697601..697912	+	312	WP_003403164.1	hypothetical protein	-
M1777_RS03180 (M1777_03180)	697984..698934	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
M1777_RS03185 (M1777_03185)	699175..699759	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
M1777_RS03190 (M1777_03190)	699761..700471	+	711	Protein_632	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T243852 WP_003403137.1 NZ_CP096845:c695312-694899 [Mycobacterium tuberculosis variant bovis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T243852 NZ_OX637964:c4258583-4258296 [Escherichia coli O25b:H4-ST131]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT243852 WP_003403139.1 NZ_CP096845:c695545-695309 [Mycobacterium tuberculosis variant bovis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT243852 NZ_OX637964:c4258309-4258007 [Escherichia coli O25b:H4-ST131]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89