Detailed information of TA system TA243832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2847575..2848212
• Replicon: chromosome
• Accession: NZ_CP096844
• Organism: Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: M1776_RS13375
• Protein ID: WP_003413180.1
• Coordinates: 2847575..2847970 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: M1776_RS13380
• Protein ID: WP_003413183.1
• Coordinates: 2847967..2848212 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1776_RS13335 (M1776_13340)	2843660..2844319	+	660	WP_273590156.1	LppA family lipoprotein	-
M1776_RS13340 (M1776_13345)	2844316..2844975	+	660	WP_003900847.1	LppA family lipoprotein	-
M1776_RS13345 (M1776_13350)	2844972..2845634	+	663	WP_003900848.1	LppA family lipoprotein	-
M1776_RS13350 (M1776_13355)	2845631..2845909	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
M1776_RS13355 (M1776_13360)	2846002..2846415	+	414	WP_003413164.1	PIN domain nuclease	-
M1776_RS13360 (M1776_13365)	2846454..2846711	+	258	WP_003413167.1	CopG family transcriptional regulator	-
M1776_RS13365 (M1776_13370)	2846708..2847085	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
M1776_RS13370 (M1776_13375)	2847101..2847475	-	375	WP_003413177.1	hypothetical protein	-
M1776_RS13375 (M1776_13380)	2847575..2847970	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
M1776_RS13380 (M1776_13385)	2847967..2848212	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
M1776_RS13385 (M1776_13390)	2848623..2849042	-	420	WP_003413190.1	A24 family peptidase	-
M1776_RS13390 (M1776_13395)	2849054..2849862	-	809	Protein_2643	shikimate dehydrogenase	-
M1776_RS13395 (M1776_13400)	2849859..2851112	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
M1776_RS13400 (M1776_13405)	2851105..2851617	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T243832 WP_003413180.1 NZ_CP096844:c2847970-2847575 [Mycobacterium tuberculosis variant bovis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T243832 NZ_OX637961:c4378372-4378199 [Escherichia coli O25b:H4-ST131]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT243832 WP_003413183.1 NZ_CP096844:c2848212-2847967 [Mycobacterium tuberculosis variant bovis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT243832 NZ_OX637961:c4378696-4378400 [Escherichia coli O25b:H4-ST131]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31