Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2256102..2257021 Replicon chromosome
Accession NZ_CP096844
Organism Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag M1776_RS10590 Protein ID WP_003900449.1
Coordinates 2256416..2257021 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag M1776_RS10585 Protein ID WP_003410124.1
Coordinates 2256102..2256407 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1776_RS10555 (M1776_10560) 2251113..2252369 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
M1776_RS10560 (M1776_10565) 2252723..2253298 + 576 WP_003410100.1 hypothetical protein -
M1776_RS10565 (M1776_10570) 2253295..2254335 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
M1776_RS10570 (M1776_10575) 2254577..2255296 + 720 WP_010950643.1 DUF433 domain-containing protein -
M1776_RS10575 (M1776_10580) 2255286..2255702 + 417 WP_003410114.1 hypothetical protein -
M1776_RS10580 (M1776_10585) 2255718..2256017 - 300 WP_003410120.1 hypothetical protein -
M1776_RS10585 (M1776_10590) 2256102..2256407 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
M1776_RS10590 (M1776_10595) 2256416..2257021 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M1776_RS10595 (M1776_10600) 2257046..2257405 - 360 WP_003410131.1 hypothetical protein -
M1776_RS10600 (M1776_10605) 2257565..2258197 - 633 WP_003913310.1 hypothetical protein -
M1776_RS10605 (M1776_10610) 2258194..2258430 - 237 WP_003410133.1 hypothetical protein -
M1776_RS10610 (M1776_10615) 2258637..2259533 - 897 WP_011799238.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T243824 WP_003900449.1 NZ_CP096844:c2257021-2256416 [Mycobacterium tuberculosis variant bovis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T243824 NZ_OX637961:2852856-2853134 [Escherichia coli O25b:H4-ST131]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT243824 WP_003410124.1 NZ_CP096844:c2256407-2256102 [Mycobacterium tuberculosis variant bovis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT243824 NZ_OX637961:2852663-2852854 [Escherichia coli O25b:H4-ST131]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References