Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2203734..2204602 Replicon chromosome
Accession NZ_CP096844
Organism Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag M1776_RS10275 Protein ID WP_010886136.1
Coordinates 2203734..2204111 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag M1776_RS10280 Protein ID WP_003409886.1
Coordinates 2204153..2204602 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1776_RS10225 (M1776_10230) 2199523..2199975 - 453 WP_010950636.1 lipoprotein -
M1776_RS10230 (M1776_10235) 2200039..2200440 + 402 WP_003409869.1 hypothetical protein -
M1776_RS10235 (M1776_10240) 2200433..2200615 - 183 WP_003409870.1 hypothetical protein -
M1776_RS10240 (M1776_10245) 2200729..2201079 - 351 WP_003409871.1 hypothetical protein -
M1776_RS10245 (M1776_10250) 2201090..2201992 - 903 WP_003409874.1 hypothetical protein -
M1776_RS10250 (M1776_10255) 2202013..2202204 - 192 WP_003409876.1 hypothetical protein -
M1776_RS10255 (M1776_10260) 2202205..2202501 - 297 WP_003409877.1 hypothetical protein -
M1776_RS10260 (M1776_10265) 2202741..2202956 + 216 WP_003409878.1 antitoxin -
M1776_RS10265 (M1776_10270) 2202953..2203264 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
M1776_RS10270 (M1776_10275) 2203238..2203759 - 522 WP_010950637.1 hypothetical protein -
M1776_RS10275 (M1776_10280) 2203734..2204111 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
M1776_RS10280 (M1776_10285) 2204153..2204602 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
M1776_RS10285 (M1776_10290) 2204599..2205144 + 546 WP_003409891.1 SecB-like chaperone -
M1776_RS10290 (M1776_10295) 2205033..2205647 - 615 WP_003901296.1 hypothetical protein -
M1776_RS10295 (M1776_10300) 2205696..2205992 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
M1776_RS10300 (M1776_10305) 2205989..2206240 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
M1776_RS10305 (M1776_10310) 2206227..2206721 + 495 WP_003899099.1 hypothetical protein -
M1776_RS10310 (M1776_10315) 2206881..2207288 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
M1776_RS10315 (M1776_10320) 2207292..2207564 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M1776_RS10320 (M1776_10325) 2207597..2208817 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T243818 WP_010886136.1 NZ_CP096844:2203734-2204111 [Mycobacterium tuberculosis variant bovis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T243818 NZ_OX637961:1902224-1902475 [Escherichia coli O25b:H4-ST131]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT243818 WP_003409886.1 NZ_CP096844:2204153-2204602 [Mycobacterium tuberculosis variant bovis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT243818 NZ_OX637961:1902472-1902726 [Escherichia coli O25b:H4-ST131]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References