Detailed information of TA system TA243794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 698036..698682
• Replicon: chromosome
• Accession: NZ_CP096844
• Organism: Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: M1776_RS03155
• Protein ID: WP_003403137.1
• Coordinates: 698036..698449 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: M1776_RS03160
• Protein ID: WP_003403139.1
• Coordinates: 698446..698682 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1776_RS03135 (M1776_03135)	694119..695669	+	1551	WP_003403119.1	MlaD family protein	-
M1776_RS03140 (M1776_03140)	695721..696113	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
M1776_RS03145 (M1776_03145)	696110..696367	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
M1776_RS03150 (M1776_03150)	696550..697785	-	1236	WP_069523374.1	ATP-binding protein	-
M1776_RS03155 (M1776_03155)	698036..698449	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1776_RS03160 (M1776_03160)	698446..698682	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
M1776_RS03165 (M1776_03165)	698786..699292	-	507	WP_003900973.1	two component system sensor kinase HK1	-
M1776_RS03170 (M1776_03170)	699406..699936	-	531	WP_003917320.1	two component system sensor kinase HK2	-
M1776_RS03175 (M1776_03175)	699920..700615	-	696	WP_197044064.1	two-component system response regulator TcrA	-
M1776_RS03180 (M1776_03180)	700738..701049	+	312	WP_003403164.1	hypothetical protein	-
M1776_RS03185 (M1776_03185)	701121..702071	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
M1776_RS03190 (M1776_03190)	702312..702896	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
M1776_RS03195 (M1776_03195)	702898..703608	+	711	Protein_633	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T243794 WP_003403137.1 NZ_CP096844:c698449-698036 [Mycobacterium tuberculosis variant bovis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T243794 NZ_OX637959:1902221-1902472 [Escherichia coli O25b:H4-ST131]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT243794 WP_003403139.1 NZ_CP096844:c698682-698446 [Mycobacterium tuberculosis variant bovis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT243794 NZ_OX637959:1902469-1902723 [Escherichia coli O25b:H4-ST131]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89