Detailed information of TA system TA024374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9823..10466
• Replicon: plasmid pO26_1
• Accession: NC_013369
• Organism: Escherichia coli O26:H11 str. 11368

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: ECO26_RS29715
• Protein ID: WP_001044768.1
• Coordinates: 10050..10466 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: ECO26_RS29710
• Protein ID: WP_001261287.1
• Coordinates: 9823..10053 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO26_RS29685	5688..7127	+	1440	WP_001213545.1	enterohemolysin T1SS ABC transporter subunit EhxD	-
ECO26_RS34475	7194..7337	+	144	Protein_4	transcriptional regulator	-
ECO26_RS34480	7341..7532	+	192	Protein_5	lipid II-degrading bacteriocin	-
ECO26_RS29695	7540..7893	-	354	WP_000864810.1	colicin M immunity protein	-
ECO26_RS29700	8066..8848	-	783	WP_001164205.1	site-specific integrase	-
ECO26_RS29705	8850..9263	-	414	WP_000465041.1	hypothetical protein	-
ECO26_RS29710	9823..10053	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ECO26_RS29715	10050..10466	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
ECO26_RS29720	10628..11173	-	546	WP_001165114.1	hypothetical protein	-
ECO26_RS33380	11241..12397	+	1157	WP_085948186.1	IS3-like element IS600 family transposase	-
ECO26_RS34930	12438..12587	-	150	WP_000955366.1	hypothetical protein	-
ECO26_RS29735	13202..14062	-	861	WP_000704534.1	alpha/beta hydrolase	-
ECO26_RS29740	14190..14576	+	387	WP_001066949.1	tyrosine-type recombinase/integrase	-
ECO26_RS29745	14630..15304	+	675	WP_001341423.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB	1..85167	85167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T24374 WP_001044768.1 NC_013369:10050-10466 [Escherichia coli O26:H11 str. 11368]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T24374 NC_013369:10050-10466 [Escherichia coli O26:H11 str. 11368]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT24374 WP_001261287.1 NC_013369:9823-10053 [Escherichia coli O26:H11 str. 11368]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT24374 NC_013369:9823-10053 [Escherichia coli O26:H11 str. 11368]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4