Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3065386..3065956 Replicon chromosome
Accession NZ_CP096841
Organism Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag M1774_RS14535 Protein ID WP_003414166.1
Coordinates 3065386..3065742 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag M1774_RS14540 Protein ID WP_003901465.1
Coordinates 3065726..3065956 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1774_RS14515 (M1774_14520) 3060838..3062526 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
M1774_RS14520 (M1774_14525) 3062530..3062856 - 327 WP_003414157.1 hypothetical protein -
M1774_RS14525 (M1774_14530) 3063029..3063616 + 588 WP_003914429.1 DUF3558 family protein -
M1774_RS14530 (M1774_14535) 3063635..3065284 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
M1774_RS14535 (M1774_14540) 3065386..3065742 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
M1774_RS14540 (M1774_14545) 3065726..3065956 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
M1774_RS14545 (M1774_14550) 3065999..3067042 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
M1774_RS14550 (M1774_14555) 3067041..3067508 + 468 WP_003414177.1 DUF1778 domain-containing protein -
M1774_RS14555 (M1774_14560) 3067684..3067938 - 255 WP_003917684.1 hypothetical protein -
M1774_RS14560 (M1774_14565) 3068086..3068490 + 405 WP_003414181.1 hypothetical protein -
M1774_RS14565 (M1774_14570) 3068487..3068678 + 192 WP_003414184.1 hypothetical protein -
M1774_RS14570 (M1774_14575) 3068895..3069155 + 261 Protein_2875 transposase -
M1774_RS14575 (M1774_14580) 3070265..3070522 + 258 WP_003899489.1 hypothetical protein -
M1774_RS14580 (M1774_14585) 3070627..3070938 + 312 WP_010950777.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T243660 WP_003414166.1 NZ_CP096841:c3065742-3065386 [Mycobacterium tuberculosis variant bovis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T243660 NZ_OX419580:c1397889-1397143 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT243660 WP_003901465.1 NZ_CP096841:c3065956-3065726 [Mycobacterium tuberculosis variant bovis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT243660 NZ_OX419580:c1397143-1396973 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References