Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2243099..2244018 Replicon chromosome
Accession NZ_CP096841
Organism Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag M1774_RS10500 Protein ID WP_003900449.1
Coordinates 2243413..2244018 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag M1774_RS10495 Protein ID WP_003410124.1
Coordinates 2243099..2243404 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1774_RS10465 (M1774_10475) 2238110..2239366 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
M1774_RS10470 (M1774_10480) 2239720..2240295 + 576 WP_003410100.1 hypothetical protein -
M1774_RS10475 (M1774_10485) 2240292..2241332 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
M1774_RS10480 (M1774_10490) 2241574..2242293 + 720 WP_010950643.1 DUF433 domain-containing protein -
M1774_RS10485 (M1774_10495) 2242283..2242699 + 417 WP_003410114.1 hypothetical protein -
M1774_RS10490 (M1774_10500) 2242715..2243014 - 300 WP_003410120.1 hypothetical protein -
M1774_RS10495 (M1774_10505) 2243099..2243404 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
M1774_RS10500 (M1774_10510) 2243413..2244018 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M1774_RS10505 (M1774_10515) 2244043..2244402 - 360 WP_003410131.1 hypothetical protein -
M1774_RS10510 (M1774_10520) 2244562..2245215 - 654 WP_014324107.1 hypothetical protein -
M1774_RS10515 (M1774_10525) 2245191..2245427 - 237 WP_003410133.1 hypothetical protein -
M1774_RS10520 (M1774_10530) 2245634..2246530 - 897 WP_003410136.1 hypothetical protein -
M1774_RS10525 (M1774_10535) 2246714..2247862 - 1149 Protein_2080 helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T243648 WP_003900449.1 NZ_CP096841:c2244018-2243413 [Mycobacterium tuberculosis variant bovis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T243648 NZ_OX419577:1867037-1867213 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTAACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGAGACAAGCAAAAAGAAAAAGCGTATACGTAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT243648 WP_003410124.1 NZ_CP096841:c2243404-2243099 [Mycobacterium tuberculosis variant bovis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT243648 NZ_OX419577:c1867096-1866996 [Bacillus subtilis]
GATTGTTAGAACCGTTAAAGATATGAGGAAAGCAACTATGATACCCACTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References