Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3027927..3028606 Replicon chromosome
Accession NZ_CP096840
Organism Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag M1780_RS14335 Protein ID WP_003414059.1
Coordinates 3027927..3028343 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag M1780_RS14340 Protein ID WP_003414061.1
Coordinates 3028340..3028606 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1780_RS14315 (M1780_14310) 3023979..3024881 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
M1780_RS14320 (M1780_14315) 3024950..3025702 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
M1780_RS14325 (M1780_14325) 3025946..3026221 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
M1780_RS14330 (M1780_14330) 3026218..3027840 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
M1780_RS14335 (M1780_14335) 3027927..3028343 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
M1780_RS14340 (M1780_14340) 3028340..3028606 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M1780_RS14345 (M1780_14345) 3028632..3029027 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
M1780_RS14350 (M1780_14350) 3029024..3029293 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
M1780_RS14355 (M1780_14355) 3029303..3030397 - 1095 WP_003414068.1 restriction endonuclease subunit S -
M1780_RS14360 (M1780_14360) 3030394..3030813 - 420 Protein_2833 winged helix-turn-helix domain-containing protein -
M1780_RS14365 (M1780_14365) 3030812..3030886 + 75 Protein_2834 hypothetical protein -
M1780_RS14370 (M1780_14370) 3030887..3031366 - 480 WP_003414073.1 dihydrofolate reductase -
M1780_RS14375 (M1780_14375) 3031437..3032237 - 801 WP_003911953.1 thymidylate synthase -
M1780_RS14380 (M1780_14380) 3032393..3033130 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T243599 WP_003414059.1 NZ_CP096840:c3028343-3027927 [Mycobacterium tuberculosis variant bovis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T243599 NZ_OX419557:c2211723-2211547 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCGTAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT243599 WP_003414061.1 NZ_CP096840:c3028606-3028340 [Mycobacterium tuberculosis variant bovis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT243599 NZ_OX419557:2211506-2211606 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References