Detailed information of TA system TA243561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 701467..702110
• Replicon: chromosome
• Accession: NZ_CP096840
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: M1780_RS03200
• Protein ID: WP_003403187.1
• Coordinates: 701709..702110 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: M1780_RS03195
• Protein ID: WP_003403184.1
• Coordinates: 701467..701712 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1780_RS03160 (M1780_03160)	696747..697277	-	531	WP_003917320.1	two component system sensor kinase HK2	-
M1780_RS03165 (M1780_03165)	697261..697956	-	696	WP_197044064.1	two-component system response regulator TcrA	-
M1780_RS03170 (M1780_03170)	698079..698390	+	312	WP_003403164.1	hypothetical protein	-
M1780_RS03175 (M1780_03175)	698462..699412	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
M1780_RS03180 (M1780_03180)	699653..700237	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
M1780_RS03185 (M1780_03185)	700239..700949	+	711	Protein_631	IS607 family element RNA-guided endonuclease TnpB	-
M1780_RS03190 (M1780_03190)	700952..701422	+	471	WP_003898523.1	hypothetical protein	-
M1780_RS03195 (M1780_03195)	701467..701712	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1780_RS03200 (M1780_03200)	701709..702110	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1780_RS03205 (M1780_03205)	702101..702280	+	180	Protein_635	hypothetical protein	-
M1780_RS03210 (M1780_03210)	702698..702895	-	198	WP_003403191.1	hypothetical protein	-
M1780_RS03215 (M1780_03215)	702975..704132	-	1158	WP_003403193.1	hypothetical protein	-
M1780_RS03220 (M1780_03220)	704184..704405	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
M1780_RS03225 (M1780_03225)	704547..705152	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T243561 WP_003403187.1 NZ_CP096840:701709-702110 [Mycobacterium tuberculosis variant bovis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T243561 NZ_OX359241:c2830092-2829796 [Alteromonas macleodii]
ATGAATAAATATACCCTCTCCAATAAAGCAGAGGAAGTTATCTCCGGGATTTACGATTATTCAGCCAATATTCATGGCGT
AACTCAAGCTGAAGCCTATCTTGTTGCCCTACACGAAGCTCTAGTCATTCTGGCGGAAAAACCTAGTATCGGTACTTCAG
CAGAGGATATCAGAAAGAATTACTTTCAACTGCCAGTTCATAGGCACATGGTCTTCTATAAAAAAGTCGATGACGGCATT
TTCGTTATTAGGGTACTTCATCAACAGATGAAATACTCTATACATCTATGCGATTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT243561 WP_003403184.1 NZ_CP096840:701467-701712 [Mycobacterium tuberculosis variant bovis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT243561 NZ_OX359241:c2830319-2830089 [Alteromonas macleodii]
ATGGCAACTTTAAATGTGTCGCTTCCTGACGAAATGCGTACGTGGATTGATGAACAAGTAAAAACTGGCAAATTTGCTAA
TGCTAGTGATTATATTCGCGATTTAGTGCGTCGCAATCAGAGTGAGCGAGAGGCAATTAGCTTAGCTCTCATAGAAGGTG
AATTGAGTGGGAAAAGTGATAAGAATGTTTTAGATATTATTCAAGTTAAGAAAACTCGAGCCTCAGAATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91