Detailed information of TA system TA243530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2240880..2241506
• Replicon: chromosome
• Accession: NZ_CP096839
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2487

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: M1779_RS10505
• Protein ID: WP_003410075.1
• Coordinates: 2241108..2241506 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: M1779_RS10500
• Protein ID: WP_019283586.1
• Coordinates: 2240880..2241107 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1779_RS10490 (M1779_10495)	2238919..2239263	-	345	WP_003410065.1	ferredoxin family protein	-
M1779_RS10495 (M1779_10500)	2239452..2240621	-	1170	WP_003899126.1	ATP-binding protein	-
M1779_RS10500 (M1779_10505)	2240880..2241107	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1779_RS10505 (M1779_10510)	2241108..2241506	+	399	WP_003410075.1	PIN domain nuclease	Toxin
M1779_RS10510 (M1779_10515)	2241689..2242120	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
M1779_RS10515 (M1779_10520)	2242221..2242655	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
M1779_RS10520 (M1779_10525)	2243092..2243271	-	180	Protein_2079	hypothetical protein	-
M1779_RS10525 (M1779_10530)	2243359..2243979	+	621	WP_003410086.1	IS110 family transposase	-
M1779_RS10530 (M1779_10535)	2243933..2244523	+	591	WP_003899131.1	IS110 family transposase	-
M1779_RS10535 (M1779_10540)	2244651..2245907	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T243530 WP_003410075.1 NZ_CP096839:2241108-2241506 [Mycobacterium tuberculosis variant bovis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T243530 NZ_OX359175:c4726646-4726113 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT243530 WP_019283586.1 NZ_CP096839:2240880-2241107 [Mycobacterium tuberculosis variant bovis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT243530 NZ_OX359175:c4726923-4726657 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• PDB: 4CHG