Detailed information of TA system TA243367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3206586..3208174
• Replicon: chromosome
• Accession: NZ_CP096810
• Organism: Klebsiella pneumoniae strain Bio19

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486S9S0
• Locus tag: MOV22_RS15555
• Protein ID: WP_023301940.1
• Coordinates: 3206586..3207908 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MOV22_RS15560
• Protein ID: WP_024622638.1
• Coordinates: 3207908..3208174 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOV22_RS15540 (3201642)	3201642..3204143	+	2502	WP_087774691.1	fimbria/pilus outer membrane usher protein	-
MOV22_RS15545 (3204131)	3204131..3205135	+	1005	WP_009308876.1	fimbrial protein	-
MOV22_RS15550 (3205277)	3205277..3206569	+	1293	WP_032423495.1	glycoside hydrolase family 10 protein	-
MOV22_RS15555 (3206586)	3206586..3207908	-	1323	WP_023301940.1	type II toxin-antitoxin system HipA family toxin	Toxin
MOV22_RS15560 (3207908)	3207908..3208174	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MOV22_RS15565 (3208589)	3208589..3208915	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
MOV22_RS15570 (3208912)	3208912..3209412	-	501	WP_048999765.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
MOV22_RS15575 (3209390)	3209390..3210769	-	1380	WP_002904785.1	nucleobase:cation symporter-2 family protein	-
MOV22_RS15580 (3211115)	3211115..3212269	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
MOV22_RS15585 (3212250)	3212250..3213173	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49185.49 Da
• Isoelectric Point: 7.5358

>T243367 WP_023301940.1 NZ_CP096810:c3207908-3206586 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T243367 NZ_OX030701:2892026-2892337 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT243367 WP_024622638.1 NZ_CP096810:c3208174-3207908 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT243367 NZ_OX030701:2892338-2892628 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A486S9S0