Detailed information of TA system TA024331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3721114..3721841
• Replicon: chromosome
• Accession: NC_013361
• Organism: Escherichia coli O26:H11 str. 11368

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: ECO26_RS19795
• Protein ID: WP_000547559.1
• Coordinates: 3721530..3721841 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECO26_RS19790
• Protein ID: WP_000126294.1
• Coordinates: 3721114..3721533 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO26_RS19770	3716164..3716691	-	528	WP_001078781.1	electron transport protein HydN	-
ECO26_RS19775	3716840..3717850	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
ECO26_RS19780	3718110..3719567	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
ECO26_RS19785	3719576..3721000	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
ECO26_RS19790	3721114..3721533	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
ECO26_RS19795	3721530..3721841	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
ECO26_RS19800	3722015..3722758	-	744	WP_001341829.1	hypothetical protein	-
ECO26_RS19805	3722801..3723271	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
ECO26_RS19810	3723264..3723674	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
ECO26_RS19815	3723671..3724438	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
ECO26_RS19820	3724438..3724980	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
ECO26_RS19825	3724990..3726699	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T24331 WP_000547559.1 NC_013361:c3721841-3721530 [Escherichia coli O26:H11 str. 11368]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T24331 NC_013361:c3721841-3721530 [Escherichia coli O26:H11 str. 11368]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT24331 WP_000126294.1 NC_013361:c3721533-3721114 [Escherichia coli O26:H11 str. 11368]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT24331 NC_013361:c3721533-3721114 [Escherichia coli O26:H11 str. 11368]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163