Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2142510..2143024 Replicon chromosome
Accession NZ_CP096539
Organism Staphylococcus aureus strain RIVM_M047065

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag M1F46_RS10535 Protein ID WP_000581792.1
Coordinates 2142510..2142719 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag M1F46_RS10540 Protein ID WP_000138932.1
Coordinates 2142731..2143024 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F46_RS10515 (M1F46_10510) 2138021..2139376 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
M1F46_RS10520 (M1F46_10515) 2139391..2140461 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
M1F46_RS10525 (M1F46_10520) 2140779..2141981 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
M1F46_RS10530 (M1F46_10525) 2142245..2142382 - 138 WP_000828354.1 Lmo0850 family protein -
M1F46_RS10535 (M1F46_10530) 2142510..2142719 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
M1F46_RS10540 (M1F46_10535) 2142731..2143024 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
M1F46_RS10545 (M1F46_10540) 2143190..2144674 + 1485 WP_000571550.1 cardiolipin synthase -
M1F46_RS10550 (M1F46_10545) 2144702..2145349 + 648 WP_001187614.1 HD domain-containing protein -
M1F46_RS10555 (M1F46_10550) 2145906..2145968 + 63 Protein_2037 hypothetical protein -
M1F46_RS10560 (M1F46_10555) 2145946..2147261 - 1316 Protein_2038 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T243142 WP_000581792.1 NZ_CP096539:c2142719-2142510 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T243142 NZ_OW970357:2052434-2052536 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT243142 WP_000138932.1 NZ_CP096539:c2143024-2142731 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT243142 NZ_OW970357:c2052543-2052398 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References