Detailed information of TA system TA243029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 176738..177260
• Replicon: plasmid pKP20-558-1
• Accession: NZ_CP096264
• Organism: Klebsiella quasipneumoniae strain KP20-558

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: M0M92_RS27335
• Protein ID: WP_014839901.1
• Coordinates: 176738..177022 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: M0M92_RS27340
• Protein ID: WP_014839900.1
• Coordinates: 177012..177260 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0M92_RS27300 (M0M92_27310)	172107..173087	+	981	WP_042934497.1	IS5-like element IS5 family transposase	-
M0M92_RS27305 (M0M92_27315)	173291..173812	+	522	WP_048336588.1	hypothetical protein	-
M0M92_RS27310 (M0M92_27320)	173809..174411	+	603	WP_014839902.1	HAD domain-containing protein	-
M0M92_RS29930	174652..175371	+	720	WP_042934502.1	cation:proton antiporter	-
M0M92_RS27325 (M0M92_27335)	175388..176065	-	678	WP_042934501.1	hypothetical protein	-
M0M92_RS27330 (M0M92_27340)	176071..176547	-	477	WP_042934526.1	hypothetical protein	-
M0M92_RS27335 (M0M92_27345)	176738..177022	-	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M0M92_RS27340 (M0M92_27350)	177012..177260	-	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
M0M92_RS27345 (M0M92_27355)	177559..179091	-	1533	WP_064386399.1	IS3 family transposase	-
M0M92_RS27350 (M0M92_27360)	179203..180606	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
M0M92_RS27355 (M0M92_27365)	180635..181267	-	633	WP_001567369.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / tet(D) / aac(3)-IId / sul2	-	1..232977	232977
	-	inside	IScluster/Tn	-	-	172107..180606	8499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T243029 WP_014839901.1 NZ_CP096264:c177022-176738 [Klebsiella quasipneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T243029 NZ_OW968328:c3941279-3940866 [Enterobacter cloacae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCGTTATTGCTCCACGGCCTGGTCGCGGC
ATTTGTTTTACTGATGCCGTGGCCGCTTAGCTATACGCCGCTGTGGCTGTTGTTGCTGTCGTTCGTCGTTTTTGACAGCG
TGCGCAGCCAGCGCCGGATCAATGCCCGCCAGGGTGAGATCAAATTACTGATGGACTCCCGCCTGCGCTGGCAGGGTCAG
GAGTGGGATATCCTCGGTATGCCCTGGATGCTCAGTTCCGGCATGATGCTACGGTTACGCCAGGTGGACGGCGGTAAGTG
CCAGCATCTGTGGCTGGCCGCCGACAGTATGGATATTGCGGAGTGGCGGGAACTGCGCCGGATGCTACTGCAGCAGCAAC
CGACGCAGGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT243029 WP_014839900.1 NZ_CP096264:c177260-177012 [Klebsiella quasipneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT243029 NZ_OW968328:c3941526-3941260 [Enterobacter cloacae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCATGCCGACGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGAGGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5