Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 5213622..5214217 Replicon chromosome
Accession NC_013353
Organism Escherichia coli O103:H2 str. 12009

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag ECO103_RS26655 Protein ID WP_000239579.1
Coordinates 5213867..5214217 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag ECO103_RS26650 Protein ID WP_001223208.1
Coordinates 5213622..5213873 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO103_RS26640 5209286..5213065 + 3780 WP_000060939.1 autotransporter assembly complex protein TamB -
ECO103_RS26645 5213068..5213409 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
ECO103_RS26650 5213622..5213873 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
ECO103_RS26655 5213867..5214217 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
ECO103_RS26660 5214297..5214827 - 531 WP_000055072.1 inorganic diphosphatase -
ECO103_RS26665 5215137..5216093 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
ECO103_RS26670 5216233..5217735 + 1503 WP_000205800.1 sugar ABC transporter ATP-binding protein -
ECO103_RS26675 5217749..5218771 + 1023 WP_001298926.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T24301 WP_000239579.1 NC_013353:5213867-5214217 [Escherichia coli O103:H2 str. 12009]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T24301 NC_013353:5213867-5214217 [Escherichia coli O103:H2 str. 12009]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT24301 WP_001223208.1 NC_013353:5213622-5213873 [Escherichia coli O103:H2 str. 12009]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT24301 NC_013353:5213622-5213873 [Escherichia coli O103:H2 str. 12009]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References