Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5848..6373 Replicon plasmid pAR-DD02201
Accession NZ_CP096240
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02201

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag M0536_RS27980 Protein ID WP_013023785.1
Coordinates 5848..6153 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag M0536_RS27985 Protein ID WP_001568025.1
Coordinates 6155..6373 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0536_RS27950 (M0536_27905) 1543..2169 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
M0536_RS27955 (M0536_27910) 2166..2468 + 303 WP_004197636.1 hypothetical protein -
M0536_RS27960 (M0536_27915) 2924..3718 - 795 WP_004197635.1 site-specific integrase -
M0536_RS27965 (M0536_27920) 3916..4932 - 1017 WP_017899884.1 hypothetical protein -
M0536_RS27970 (M0536_27925) 4943..5257 - 315 WP_053389906.1 hypothetical protein -
M0536_RS27975 (M0536_27930) 5284..5679 - 396 WP_017899885.1 hypothetical protein -
M0536_RS27980 (M0536_27935) 5848..6153 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
M0536_RS27985 (M0536_27940) 6155..6373 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
M0536_RS27990 (M0536_27945) 7004..7201 + 198 WP_017899886.1 hypothetical protein -
M0536_RS27995 (M0536_27950) 7191..7481 - 291 WP_013023783.1 hypothetical protein -
M0536_RS28000 (M0536_27955) 7478..8605 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
M0536_RS28005 (M0536_27960) 8639..10162 - 1524 WP_017899887.1 hypothetical protein -
M0536_RS28010 (M0536_27965) 10438..11217 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qnrB52 - 1..97079 97079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T242954 WP_013023785.1 NZ_CP096240:c6153-5848 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242954 NZ_OW968124:2721197-2721304 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT242954 WP_001568025.1 NZ_CP096240:c6373-6155 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242954 NZ_OW968124:c2721150-2721084 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References