Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3377789..3378372 Replicon chromosome
Accession NC_013353
Organism Escherichia coli O103:H2 str. 12009

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag ECO103_RS17515 Protein ID WP_000254745.1
Coordinates 3377789..3378124 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag ECO103_RS17520 Protein ID WP_000581937.1
Coordinates 3378124..3378372 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO103_RS17500 3373676..3374974 - 1299 WP_000036723.1 phosphopyruvate hydratase -
ECO103_RS17505 3375062..3376699 - 1638 WP_000210883.1 CTP synthase (glutamine hydrolyzing) -
ECO103_RS17510 3376927..3377718 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
ECO103_RS17515 3377789..3378124 - 336 WP_000254745.1 endoribonuclease MazF Toxin
ECO103_RS17520 3378124..3378372 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ECO103_RS17525 3378450..3380684 - 2235 WP_000226815.1 GTP pyrophosphokinase -
ECO103_RS17530 3380732..3382033 - 1302 WP_000046800.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T24292 WP_000254745.1 NC_013353:c3378124-3377789 [Escherichia coli O103:H2 str. 12009]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T24292 NC_013353:c3378124-3377789 [Escherichia coli O103:H2 str. 12009]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT24292 WP_000581937.1 NC_013353:c3378372-3378124 [Escherichia coli O103:H2 str. 12009]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT24292 NC_013353:c3378372-3378124 [Escherichia coli O103:H2 str. 12009]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References