Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 488496..489133 Replicon chromosome
Accession NZ_CP096033
Organism Bacillus amyloliquefaciens strain GL18

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag MY487_RS02510 Protein ID WP_003156187.1
Coordinates 488783..489133 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag MY487_RS02505 Protein ID WP_003156188.1
Coordinates 488496..488777 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY487_RS02485 (MY487_02485) 484861..485460 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
MY487_RS02490 (MY487_02490) 485553..485918 + 366 WP_012116869.1 holo-ACP synthase -
MY487_RS02495 (MY487_02495) 486083..487090 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
MY487_RS02500 (MY487_02500) 487207..488376 + 1170 WP_012116870.1 alanine racemase -
MY487_RS02505 (MY487_02505) 488496..488777 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
MY487_RS02510 (MY487_02510) 488783..489133 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MY487_RS02515 (MY487_02515) 489251..490072 + 822 WP_003156182.1 STAS domain-containing protein -
MY487_RS02520 (MY487_02520) 490077..490442 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
MY487_RS02525 (MY487_02525) 490445..490846 + 402 WP_003156178.1 anti-sigma regulatory factor -
MY487_RS02530 (MY487_02530) 490858..491865 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
MY487_RS02535 (MY487_02535) 491929..492258 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
MY487_RS02540 (MY487_02540) 492255..492737 + 483 WP_007609591.1 anti-sigma B factor RsbW -
MY487_RS02545 (MY487_02545) 492703..493491 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
MY487_RS02550 (MY487_02550) 493491..494093 + 603 WP_012116871.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T242721 WP_003156187.1 NZ_CP096033:488783-489133 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T242721 NZ_OW967003:1040976-1041083 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT242721 WP_003156188.1 NZ_CP096033:488496-488777 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT242721 NZ_OW967003:c1040923-1040862 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References