Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2033323..2033977 Replicon chromosome
Accession NZ_CP095856
Organism Escherichia coli strain EC6563

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag MYG04_RS09870 Protein ID WP_000244783.1
Coordinates 2033323..2033730 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MYG04_RS09875 Protein ID WP_000354046.1
Coordinates 2033711..2033977 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYG04_RS09850 (2029280) 2029280..2031013 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
MYG04_RS09855 (2031019) 2031019..2031729 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MYG04_RS09860 (2031754) 2031754..2032650 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MYG04_RS09865 (2032762) 2032762..2033283 + 522 WP_001055874.1 flavodoxin FldB -
MYG04_RS09870 (2033323) 2033323..2033730 - 408 WP_000244783.1 protein YgfX Toxin
MYG04_RS09875 (2033711) 2033711..2033977 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MYG04_RS09880 (2034220) 2034220..2035200 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MYG04_RS09885 (2035396) 2035396..2036055 - 660 WP_000250274.1 hemolysin III family protein -
MYG04_RS09890 (2036219) 2036219..2036530 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
MYG04_RS09895 (2036575) 2036575..2038008 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
MYG04_RS09900 (2038065) 2038065..2038808 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T242641 WP_000244783.1 NZ_CP095856:c2033730-2033323 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T242641 NZ_OW849082:c781324-780947 [Citrobacter freundii]
GTGCAAACCCAACCACTATCCTCCACGCATGAGACCTCACCACGTCCGTCCCCCGTGGATATCTGGCAGCACCTCCTGAA
CCATCTGCTGGATCGTCACTACGGTCTGACGCTAAACGACACGCCATTTGGCAACGATGGCGTGATCCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAACTTTATCGTGGAAAAGTACGACCTGGTCCGTACTGACAGACGCGGCTTT
AGCGCAGACACACAGTCACCGCTTATCGGCAGTATTGATATCCTCCGAGCCCGGAAGGCGACCGGGCTGATGACCCGTCA
TGGCTACAGACCTGTGACAGATCTCATTACCGGCAAATACAAAAAGGAACAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT242641 WP_000354046.1 NZ_CP095856:c2033977-2033711 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT242641 NZ_OW849082:c781735-781376 [Citrobacter freundii]
TTGTCGAATAAAATACCGGCAGAAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGTGCCCGTCTGGTGCAGGAGGGCAACCGGCTGCACTATCTCGGCGACCGCGCGAGTATCGCCGGCACGTTCAGCGACG
CGGATTTACGCCATCTTGACCAGGCGTTTCCGGTACAGCTCAAACAGCTTGAGCTGATGCTGGTTTCCGGTGAACTCAAT
CCCCGCCATCAGCACTGCGTCACATTATACTCAAAAGGGCTGACCTGCGAGGCCGACAGCCTCGGCTCGCATGGATACGT
GTACATCGCGATTTACCCGACACCTGCAGCAACCGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References