Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1640990..1641717 Replicon chromosome
Accession NZ_CP095540
Organism Escherichia coli strain GN03204

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MW399_RS07880 Protein ID WP_000550189.1
Coordinates 1640990..1641304 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MW399_RS07885 Protein ID WP_000560269.1
Coordinates 1641301..1641717 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW399_RS07860 (1637147) 1637147..1638133 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
MW399_RS07865 (1638212) 1638212..1638904 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
MW399_RS07870 (1638981) 1638981..1639484 - 504 WP_001295542.1 M48 family metallopeptidase -
MW399_RS07875 (1639569) 1639569..1640705 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MW399_RS07880 (1640990) 1640990..1641304 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MW399_RS07885 (1641301) 1641301..1641717 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MW399_RS07890 (1641762) 1641762..1643780 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
MW399_RS07895 (1644006) 1644006..1646357 - 2352 WP_000695431.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T242387 WP_000550189.1 NZ_CP095540:1640990-1641304 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T242387 NZ_OW052571:2000740-2001174 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT242387 WP_000560269.1 NZ_CP095540:1641301-1641717 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT242387 NZ_OW052571:2000479-2000733 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References