Detailed information of TA system TA242372

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 4098485..4098897
• Replicon: chromosome
• Accession: NZ_CP095538
• Organism: Escherichia coli strain GN04592

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: MW396_RS20155
• Protein ID: WP_000132601.1
• Coordinates: 4098556..4098897 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4098485..4098561 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW396_RS20145 (4095348)	4095348..4096937	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
MW396_RS20150 (4096934)	4096934..4098328	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
- (4098485)	4098485..4098561	-	77	NuclAT_12	-	Antitoxin
- (4098485)	4098485..4098561	-	77	NuclAT_12	-	Antitoxin
- (4098485)	4098485..4098561	-	77	NuclAT_12	-	Antitoxin
- (4098485)	4098485..4098561	-	77	NuclAT_12	-	Antitoxin
- (4098485)	4098485..4098561	-	77	NuclAT_13	-	Antitoxin
- (4098485)	4098485..4098561	-	77	NuclAT_13	-	Antitoxin
- (4098485)	4098485..4098561	-	77	NuclAT_13	-	Antitoxin
- (4098485)	4098485..4098561	-	77	NuclAT_13	-	Antitoxin
MW396_RS20155 (4098556)	4098556..4098897	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
MW396_RS20160 (4099059)	4099059..4100438	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
MW396_RS20165 (4100438)	4100438..4101484	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T242372 WP_000132601.1 NZ_CP095538:4098556-4098897 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T242372 NZ_OW052571:c1182952-1182509 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT242372 NZ_CP095538:c4098561-4098485 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin