Detailed information of TA system TA242351

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 480570..481404
• Replicon: chromosome
• Accession: NZ_CP095538
• Organism: Escherichia coli strain GN04592

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: MW396_RS02400
• Protein ID: WP_000854689.1
• Coordinates: 480570..480947 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0IVP8
• Locus tag: MW396_RS02405
• Protein ID: WP_001285599.1
• Coordinates: 481024..481404 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW396_RS02370 (476964)	476964..477134	-	171	Protein_462	IS110 family transposase	-
MW396_RS02375 (477551)	477551..478484	-	934	Protein_463	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MW396_RS02380 (478477)	478477..478872	-	396	WP_000208381.1	DUF6088 family protein	-
MW396_RS02385 (478941)	478941..479786	-	846	WP_001446750.1	DUF4942 domain-containing protein	-
MW396_RS02390 (479871)	479871..480068	-	198	WP_000839250.1	DUF957 domain-containing protein	-
MW396_RS02395 (480085)	480085..480573	-	489	WP_000761698.1	DUF5983 family protein	-
MW396_RS02400 (480570)	480570..480947	-	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
MW396_RS02405 (481024)	481024..481404	-	381	WP_001285599.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MW396_RS02410 (481454)	481454..482098	-	645	WP_000086757.1	hypothetical protein	-
MW396_RS02415 (482117)	482117..482338	-	222	WP_000692315.1	DUF987 domain-containing protein	-
MW396_RS02420 (482401)	482401..482877	-	477	WP_001186761.1	RadC family protein	-
MW396_RS02425 (482893)	482893..483366	-	474	WP_001538139.1	antirestriction protein	-
MW396_RS02430 (483630)	483630..484451	-	822	WP_001234555.1	DUF932 domain-containing protein	-
MW396_RS02435 (484630)	484630..484719	-	90	WP_225385657.1	DUF905 family protein	-
MW396_RS02440 (484862)	484862..485317	-	456	WP_001309747.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	476964..477119	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T242351 WP_000854689.1 NZ_CP095538:c480947-480570 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T242351 NZ_OW052570:c3492366-3492103 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13984.78 Da
• Isoelectric Point: 5.0823

>AT242351 WP_001285599.1 NZ_CP095538:c481404-481024 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLLQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT242351 NZ_OW052570:c3492651-3492370 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA