Detailed information of TA system TA242344

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4480595..4481430
• Replicon: chromosome
• Accession: NZ_CP095533
• Organism: Escherichia coli strain GN02461

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: MW400_RS21030
• Protein ID: WP_001094445.1
• Coordinates: 4481053..4481430 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MW400_RS21025
• Protein ID: WP_024174331.1
• Coordinates: 4480595..4480963 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW400_RS21000 (4478020)	4478020..4478253	+	234	WP_001117568.1	DUF905 family protein	-
MW400_RS21005 (4478344)	4478344..4479162	+	819	WP_001234749.1	DUF932 domain-containing protein	-
MW400_RS21010 (4479254)	4479254..4479739	+	486	WP_000214415.1	antirestriction protein	-
MW400_RS21015 (4479755)	4479755..4480231	+	477	WP_001186725.1	RadC family protein	-
MW400_RS21020 (4480300)	4480300..4480521	+	222	WP_000692346.1	DUF987 domain-containing protein	-
MW400_RS21025 (4480595)	4480595..4480963	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MW400_RS21030 (4481053)	4481053..4481430	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
MW400_RS21035 (4481427)	4481427..4481915	+	489	WP_000761718.1	DUF5983 family protein	-
MW400_RS21040 (4481927)	4481927..4482124	+	198	WP_000838536.1	DUF957 domain-containing protein	-
MW400_RS21045 (4482209)	4482209..4483051	+	843	WP_059331797.1	DUF4942 domain-containing protein	-
MW400_RS21050 (4483393)	4483393..4483563	+	171	Protein_4102	IS110 family transposase	-
MW400_RS21055 (4484374)	4484374..4485357	+	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T242344 WP_001094445.1 NZ_CP095533:4481053-4481430 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T242344 NZ_OW052570:c2185004-2184603 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT242344 WP_024174331.1 NZ_CP095533:4480595-4480963 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT242344 NZ_OW052570:c2185236-2184997 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Toxin

• AlphaFold DB: A0A454A7U6