Detailed information of TA system TA242313

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3893430..3894084
• Replicon: chromosome
• Accession: NZ_CP095529
• Organism: Escherichia coli strain GN06156

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: MW397_RS18495
• Protein ID: WP_000244781.1
• Coordinates: 3893430..3893837 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: S1PPB1
• Locus tag: MW397_RS18500
• Protein ID: WP_000354046.1
• Coordinates: 3893818..3894084 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW397_RS18475 (3889387)	3889387..3891120	-	1734	WP_029305333.1	single-stranded-DNA-specific exonuclease RecJ	-
MW397_RS18480 (3891126)	3891126..3891836	-	711	WP_000715213.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
MW397_RS18485 (3891861)	3891861..3892757	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
MW397_RS18490 (3892869)	3892869..3893390	+	522	WP_001055872.1	flavodoxin FldB	-
MW397_RS18495 (3893430)	3893430..3893837	-	408	WP_000244781.1	protein YgfX	Toxin
MW397_RS18500 (3893818)	3893818..3894084	-	267	WP_000354046.1	FAD assembly factor SdhE	Antitoxin
MW397_RS18505 (3894327)	3894327..3895307	+	981	WP_029305332.1	tRNA-modifying protein YgfZ	-
MW397_RS18510 (3895384)	3895384..3896043	-	660	WP_000250269.1	hemolysin III family protein	-
MW397_RS18515 (3896207)	3896207..3896518	-	312	WP_001182949.1	N(4)-acetylcytidine aminohydrolase	-
MW397_RS18520 (3896563)	3896563..3897996	+	1434	WP_001389468.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T242313 WP_000244781.1 NZ_CP095529:c3893837-3893430 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T242313 NZ_OW052570:c55685-55308 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10547.12 Da
• Isoelectric Point: 5.1599

>AT242313 WP_000354046.1 NZ_CP095529:c3894084-3893818 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT242313 NZ_OW052570:c55266-54817 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Structures

Toxin

• AlphaFold DB: S1PAM6

Antitoxin

• PDB: 6B58
• PDB: 1X6I
• PDB: 1X6J
• PDB: 6C12
• AlphaFold DB: A0A7U9QD57