Detailed information of TA system TA242285

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 544574..545406
• Replicon: chromosome
• Accession: NZ_CP095529
• Organism: Escherichia coli strain GN06156

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A836ZBT7
• Locus tag: MW397_RS02525
• Protein ID: WP_001598217.1
• Coordinates: 545032..545406 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MW397_RS02520
• Protein ID: WP_001598216.1
• Coordinates: 544574..544942 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW397_RS02500 (542317)	542317..543135	+	819	WP_001598214.1	DUF932 domain-containing protein	-
MW397_RS02505 (543227)	543227..543712	+	486	WP_000206667.1	antirestriction protein	-
MW397_RS02510 (543728)	543728..544204	+	477	WP_032198294.1	RadC family protein	-
MW397_RS02515 (544273)	544273..544494	+	222	WP_000692303.1	DUF987 domain-containing protein	-
MW397_RS02520 (544574)	544574..544942	+	369	WP_001598216.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MW397_RS02525 (545032)	545032..545406	+	375	WP_001598217.1	TA system toxin CbtA family protein	Toxin
MW397_RS02530 (545403)	545403..545894	+	492	WP_001598218.1	DUF5983 family protein	-
MW397_RS02535 (545906)	545906..546103	+	198	WP_024192776.1	DUF957 domain-containing protein	-
MW397_RS02540 (546188)	546188..546337	+	150	Protein_476	hypothetical protein	-
MW397_RS02545 (546595)	546595..546888	+	294	Protein_477	PTS ascorbate transporter subunit IIA	-
MW397_RS02550 (546902)	546902..547552	+	651	WP_000056771.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
MW397_RS02555 (547562)	547562..548416	+	855	WP_000949501.1	L-ribulose-5-phosphate 3-epimerase UlaE	-
MW397_RS02560 (548416)	548416..549102	+	687	WP_001170835.1	L-ribulose-5-phosphate 4-epimerase UlaF	-
MW397_RS02565 (549232)	549232..549507	-	276	WP_000492918.1	DUF1471 family protein YjfY	-
MW397_RS02570 (549834)	549834..550229	+	396	WP_001216677.1	30S ribosomal protein S6	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13990.98 Da
• Isoelectric Point: 7.8045

>T242285 WP_001598217.1 NZ_CP095529:545032-545406 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLGRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T242285 NZ_OW052302:c1968581-1968279 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCACGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13605.40 Da
• Isoelectric Point: 7.3992

>AT242285 WP_001598216.1 NZ_CP095529:544574-544942 [Escherichia coli]
VSDTLSGTTHPDDNNSHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSRGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT242285 NZ_OW052302:c1968805-1968578 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCAGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A836ZBT7