Detailed information of TA system TA242281

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43954..44377
• Replicon: plasmid p5505-2
• Accession: NZ_CP095528
• Organism: Escherichia coli strain GN05505

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MW395_RS26425
• Protein ID: WP_001372321.1
• Coordinates: 43954..44079 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	44156..44377 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW395_RS26395	39331..40020	-	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-
MW395_RS26400	40207..40590	-	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MW395_RS26405	40911..41513	+	603	Protein_50	transglycosylase SLT domain-containing protein	-
MW395_RS26410	41810..42631	-	822	WP_001234445.1	DUF932 domain-containing protein	-
MW395_RS26415	42742..43038	-	297	WP_001272251.1	hypothetical protein	-
MW395_RS26420	43338..43635	+	298	Protein_53	hypothetical protein	-
MW395_RS26425	43954..44079	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MW395_RS26430	44021..44170	-	150	Protein_55	plasmid maintenance protein Mok	-
-	44156..44377	+	222	-	-	Antitoxin
-	44156..44380	-	225	NuclAT_0	-	-
-	44156..44380	-	225	NuclAT_0	-	-
-	44156..44380	-	225	NuclAT_0	-	-
-	44156..44380	-	225	NuclAT_0	-	-
MW395_RS26435	44349..45111	-	763	Protein_56	plasmid SOS inhibition protein A	-
MW395_RS26440	45108..45542	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MW395_RS26445	45611..47575	-	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
MW395_RS26450	47636..47869	-	234	WP_000006003.1	DUF905 family protein	-
MW395_RS26455	47927..48454	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
MW395_RS26460	48756..49199	+	444	Protein_61	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..72021	72021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T242281 WP_001372321.1 NZ_CP095528:c44079-43954 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T242281 NZ_OW052302:1690105-1690518 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Nucleotide

• Length: 222 bp

>AT242281 NZ_CP095528:44156-44377 [Escherichia coli]
ATGTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTTTCTGCCACACA
ACACGGTAACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGC
TGACCACACTCACTTTTCCTGAAAATAACCCGCTCATTCAGGCTGTTTACGGGTAATCTGTG

Structures

Antitoxin