Detailed information of TA system TA242277

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3367..3620
• Replicon: plasmid p5505-2
• Accession: NZ_CP095528
• Organism: Escherichia coli strain GN05505

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: MW395_RS26185
• Protein ID: WP_001336447.1
• Coordinates: 3367..3516 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	3564..3620 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW395_RS26155 (43)	43..342	-	300	WP_001528606.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MW395_RS26160 (332)	332..595	-	264	WP_001089473.1	hypothetical protein	-
MW395_RS26165 (615)	615..752	-	138	Protein_2	XRE family transcriptional regulator	-
MW395_RS26170 (1665)	1665..2522	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
MW395_RS26175 (2515)	2515..2589	-	75	WP_001365705.1	RepA leader peptide Tap	-
MW395_RS26180 (2825)	2825..3082	-	258	WP_000084404.1	replication regulatory protein RepA	-
MW395_RS26185 (3367)	3367..3516	-	150	WP_001336447.1	Hok/Gef family protein	Toxin
- (3564)	3564..3620	+	57	NuclAT_1	-	Antitoxin
- (3564)	3564..3620	+	57	NuclAT_1	-	Antitoxin
- (3564)	3564..3620	+	57	NuclAT_1	-	Antitoxin
- (3564)	3564..3620	+	57	NuclAT_1	-	Antitoxin
MW395_RS26190 (3715)	3715..4152	-	438	WP_000872609.1	hypothetical protein	-
MW395_RS26195 (4305)	4305..4523	-	219	WP_023150739.1	hypothetical protein	-
MW395_RS26200 (4792)	4792..5253	-	462	WP_019842128.1	thermonuclease family protein	-
MW395_RS26205 (5995)	5995..6198	-	204	WP_001336517.1	hypothetical protein	-
MW395_RS26210 (6350)	6350..6907	-	558	WP_000139329.1	fertility inhibition protein FinO	-
MW395_RS26215 (6962)	6962..7708	-	747	WP_019842127.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..72021	72021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T242277 WP_001336447.1 NZ_CP095528:c3516-3367 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T242277 NZ_OW052302:c1627258-1626857 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Nucleotide

• Length: 57 bp

>AT242277 NZ_CP095528:3564-3620 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin