Detailed information of TA system TA242265

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 3423387..3423799
• Replicon: chromosome
• Accession: NZ_CP095526
• Organism: Escherichia coli strain GN05505

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: MW395_RS17600
• Protein ID: WP_000132601.1
• Coordinates: 3423458..3423799 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	3423387..3423463 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW395_RS17590 (3420250)	3420250..3421839	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
MW395_RS17595 (3421836)	3421836..3423230	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
- (3423387)	3423387..3423463	-	77	NuclAT_12	-	Antitoxin
- (3423387)	3423387..3423463	-	77	NuclAT_12	-	Antitoxin
- (3423387)	3423387..3423463	-	77	NuclAT_12	-	Antitoxin
- (3423387)	3423387..3423463	-	77	NuclAT_12	-	Antitoxin
- (3423387)	3423387..3423463	-	77	NuclAT_13	-	Antitoxin
- (3423387)	3423387..3423463	-	77	NuclAT_13	-	Antitoxin
- (3423387)	3423387..3423463	-	77	NuclAT_13	-	Antitoxin
- (3423387)	3423387..3423463	-	77	NuclAT_13	-	Antitoxin
MW395_RS17600 (3423458)	3423458..3423799	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
MW395_RS17605 (3423961)	3423961..3425340	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
MW395_RS17610 (3425340)	3425340..3426386	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T242265 WP_000132601.1 NZ_CP095526:3423458-3423799 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T242265 NZ_OW052302:c944851-944420 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT242265 NZ_CP095526:c3423463-3423387 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin