Detailed information of TA system TA242244

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57199..57452
• Replicon: plasmid p4171-2
• Accession: NZ_CP095524
• Organism: Escherichia coli strain GN04171

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: MW404_RS25250
• Protein ID: WP_001336447.1
• Coordinates: 57303..57452 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	57199..57255 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW404_RS25220 (53111)	53111..53857	+	747	WP_019842127.1	conjugal transfer pilus acetylase TraX	-
MW404_RS25225 (53912)	53912..54469	+	558	WP_000139329.1	fertility inhibition protein FinO	-
MW404_RS25230 (54621)	54621..54824	+	204	WP_001336517.1	hypothetical protein	-
MW404_RS25235 (55566)	55566..56027	+	462	WP_019842128.1	thermonuclease family protein	-
MW404_RS25240 (56296)	56296..56514	+	219	WP_023150739.1	hypothetical protein	-
MW404_RS25245 (56667)	56667..57104	+	438	WP_000872609.1	hypothetical protein	-
- (57199)	57199..57255	-	57	NuclAT_1	-	Antitoxin
- (57199)	57199..57255	-	57	NuclAT_1	-	Antitoxin
- (57199)	57199..57255	-	57	NuclAT_1	-	Antitoxin
- (57199)	57199..57255	-	57	NuclAT_1	-	Antitoxin
MW404_RS25250 (57303)	57303..57452	+	150	WP_001336447.1	Hok/Gef family protein	Toxin
MW404_RS25255 (57737)	57737..57994	+	258	WP_000084404.1	replication regulatory protein RepA	-
MW404_RS25260 (58230)	58230..58304	+	75	WP_001365705.1	RepA leader peptide Tap	-
MW404_RS25265 (58297)	58297..59154	+	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
MW404_RS25270 (60067)	60067..60204	+	138	Protein_78	XRE family transcriptional regulator	-
MW404_RS25275 (60224)	60224..60487	+	264	WP_001089473.1	hypothetical protein	-
MW404_RS25280 (60477)	60477..60776	+	300	WP_001528606.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MW404_RS25285 (60812)	60812..61477	-	666	WP_001535733.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..74318	74318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T242244 WP_001336447.1 NZ_CP095524:57303-57452 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T242244 NZ_OW052189:c4063962-4063543 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT242244 NZ_CP095524:c57255-57199 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin