Detailed information of TA system TA242159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4450157..4450956
• Replicon: chromosome
• Accession: NZ_CP095514
• Organism: Escherichia coli strain GN02323

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: MW403_RS21215
• Protein ID: WP_000347252.1
• Coordinates: 4450492..4450956 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: MW403_RS21210
• Protein ID: WP_001296435.1
• Coordinates: 4450157..4450492 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW403_RS21195 (4445942)	4445942..4446712	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
MW403_RS21200 (4446728)	4446728..4448062	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
MW403_RS21205 (4448437)	4448437..4450008	+	1572	WP_001273940.1	galactarate dehydratase	-
MW403_RS21210 (4450157)	4450157..4450492	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
MW403_RS21215 (4450492)	4450492..4450956	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
MW403_RS21220 (4451011)	4451011..4451820	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
MW403_RS21225 (4452069)	4452069..4453349	+	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
MW403_RS21230 (4453372)	4453372..4453845	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
MW403_RS21235 (4453856)	4453856..4454635	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
MW403_RS21240 (4454625)	4454625..4455503	+	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
MW403_RS21245 (4455521)	4455521..4455955	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T242159 WP_000347252.1 NZ_CP095514:4450492-4450956 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T242159 NZ_OW052188:c2063581-2063171 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT242159 WP_001296435.1 NZ_CP095514:4450157-4450492 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT242159 NZ_OW052188:c2063856-2063578 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5