Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3063036..3063538 Replicon chromosome
Accession NZ_CP095514
Organism Escherichia coli strain GN02323

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag MW403_RS14780 Protein ID WP_000767822.1
Coordinates 3063036..3063290 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MW403_RS14785 Protein ID WP_001259255.1
Coordinates 3063287..3063538 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW403_RS14760 (3059529) 3059529..3060887 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
MW403_RS14765 (3060877) 3060877..3060939 - 63 WP_010723108.1 membrane protein YoeI -
MW403_RS14770 (3061154) 3061154..3062083 - 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
MW403_RS14775 (3062129) 3062129..3062953 - 825 WP_000754733.1 SDR family oxidoreductase -
MW403_RS14780 (3063036) 3063036..3063290 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MW403_RS14785 (3063287) 3063287..3063538 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MW403_RS14790 (3063821) 3063821..3063871 + 51 WP_001364200.1 his operon leader peptide -
MW403_RS14795 (3064017) 3064017..3064916 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
MW403_RS14800 (3064922) 3064922..3066226 + 1305 WP_001297912.1 histidinol dehydrogenase -
MW403_RS14805 (3066223) 3066223..3067293 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
MW403_RS14810 (3067293) 3067293..3068360 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T242155 WP_000767822.1 NZ_CP095514:c3063290-3063036 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T242155 NZ_OW052188:c1112725-1112300 [Mycobacterium tuberculosis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT242155 WP_001259255.1 NZ_CP095514:c3063538-3063287 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT242155 NZ_OW052188:c1112943-1112722 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References