Detailed information of TA system TA242028

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4278034..4278446
• Replicon: chromosome
• Accession: NZ_CP095448
• Organism: Escherichia coli strain LS45

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: MWN28_RS20800
• Protein ID: WP_000132601.1
• Coordinates: 4278105..4278446 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4278034..4278110 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWN28_RS20790 (4274897)	4274897..4276486	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
MWN28_RS20795 (4276483)	4276483..4277877	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
- (4278034)	4278034..4278110	-	77	NuclAT_8	-	Antitoxin
- (4278034)	4278034..4278110	-	77	NuclAT_8	-	Antitoxin
- (4278034)	4278034..4278110	-	77	NuclAT_8	-	Antitoxin
- (4278034)	4278034..4278110	-	77	NuclAT_8	-	Antitoxin
- (4278034)	4278034..4278110	-	77	NuclAT_9	-	Antitoxin
- (4278034)	4278034..4278110	-	77	NuclAT_9	-	Antitoxin
- (4278034)	4278034..4278110	-	77	NuclAT_9	-	Antitoxin
- (4278034)	4278034..4278110	-	77	NuclAT_9	-	Antitoxin
MWN28_RS20800 (4278105)	4278105..4278446	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
MWN28_RS20805 (4278608)	4278608..4279987	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
MWN28_RS20810 (4279987)	4279987..4281033	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4276483..4291400	14917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T242028 WP_000132601.1 NZ_CP095448:4278105-4278446 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T242028 NZ_OU963893:1064541-1064951 [unidentified bacterial endosymbiont]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCATTAATGCTCCACGGCGTGGTTGCGGC
CTTAGTTTTACTGATGCCGTGGCCGCTTAGCTACACCCCTGTATGGTTGCTTATGCTATCGTTTGTGGTTTTTGACAGCG
TGCGTAGCCAGCGTCGAATCAACGCTCGTCAGGGTGAAATCAAATTACTGGTGGATTCTCGTCTGCGCTGGCAGGGCAAG
GAGTGGGAGATCGTTGGAATGCCCTGGATGCTCCGCTCAGGCATGATGCTACGGTTACGTAACGCGGGCGGTGGGCGTCG
CCAGCATCTTTGGCTGGCCTCAGACAGCATGGATATCGCCGAGTGGCGAGATCTGCGTCGCACCTTTTTGCAGCAGCCGA
CGCAGGAATGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT242028 NZ_CP095448:c4278110-4278034 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin