Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 123097..123740 Replicon plasmid pIM057_KPC
Accession NZ_CP095423
Organism Klebsiella pneumoniae strain IM057

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MWR70_RS29210 Protein ID WP_001044770.1
Coordinates 123324..123740 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MWR70_RS29205 Protein ID WP_001261282.1
Coordinates 123097..123327 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR70_RS29170 (118320) 118320..119099 - 780 WP_013214009.1 site-specific integrase -
MWR70_RS29175 (119283) 119283..120287 - 1005 WP_011977814.1 hypothetical protein -
MWR70_RS29180 (120317) 120317..120520 - 204 WP_011977813.1 hypothetical protein -
MWR70_RS29185 (120566) 120566..121087 - 522 WP_013214008.1 hypothetical protein -
MWR70_RS29190 (121145) 121145..121537 - 393 WP_011977811.1 hypothetical protein -
MWR70_RS29195 (121574) 121574..122518 - 945 WP_011977810.1 hypothetical protein -
MWR70_RS29200 (122679) 122679..123140 - 462 WP_014343465.1 hypothetical protein -
MWR70_RS29205 (123097) 123097..123327 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MWR70_RS29210 (123324) 123324..123740 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MWR70_RS29215 (123814) 123814..125376 + 1563 WP_004206609.1 AAA family ATPase -
MWR70_RS29220 (125361) 125361..126383 + 1023 WP_000361404.1 helicase UvrD -
MWR70_RS29225 (126639) 126639..127336 + 698 WP_223174630.1 IS1 family transposase -
MWR70_RS29230 (127365) 127365..127637 + 273 Protein_175 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / blaCTX-M-65 / catA2 - 1..129505 129505


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T241947 WP_001044770.1 NZ_CP095423:123324-123740 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T241947 NZ_OU701452:c1478846-1478652 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT241947 WP_001261282.1 NZ_CP095423:123097-123327 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT241947 NZ_OU701452:1478475-1478653 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References