Detailed information of TA system TA024186

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1747676..1747901
• Replicon: chromosome
• Accession: NC_013008
• Organism: Escherichia coli O157:H7 str. TW14359

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ECSP_RS08970
• Protein ID: WP_000813254.1
• Coordinates: 1747746..1747901 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1747676..1747734 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSP_RS33000	1743130..1744170	+	1041	WP_000020570.1	DNA-binding protein	-
ECSP_RS33005	1744082..1744624	+	543	WP_157837342.1	replication protein	-
ECSP_RS08940	1744659..1745429	+	771	WP_000537576.1	DUF1627 domain-containing protein	-
ECSP_RS08945	1745445..1745840	+	396	WP_001118161.1	DUF977 family protein	-
ECSP_RS08950	1745897..1746253	+	357	WP_001302146.1	hypothetical protein	-
ECSP_RS08955	1746302..1746514	+	213	WP_000063625.1	hypothetical protein	-
ECSP_RS08960	1746550..1746921	+	372	WP_000137941.1	hypothetical protein	-
ECSP_RS08965	1746918..1747280	+	363	WP_000610377.1	DUF551 domain-containing protein	-
ECSP_RS32025	1747396..1747500	+	105	WP_001278450.1	hypothetical protein	-
-	1747676..1747734	-	59	-	-	Antitoxin
ECSP_RS08970	1747746..1747901	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
ECSP_RS08975	1748122..1748379	+	258	WP_001217394.1	hypothetical protein	-
ECSP_RS32030	1748449..1748727	+	279	WP_012779355.1	hypothetical protein	-
ECSP_RS08985	1748729..1749778	+	1050	WP_001265156.1	DUF968 domain-containing protein	-
ECSP_RS08990	1749791..1750165	+	375	WP_000904171.1	RusA family crossover junction endodeoxyribonuclease	-
ECSP_RS08995	1750162..1750983	+	822	WP_000762902.1	antitermination protein	-
ECSP_RS09000	1751210..1751407	+	198	WP_000917735.1	hypothetical protein	-
ECSP_RS09005	1751558..1752616	+	1059	WP_000483509.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / nleH2 / nleF / espM1 / ospG	1672330..1783045	110715
	-	inside	Prophage	-	paa / nleA/espI / nleH2 / nleF / espM1 / ospG	1676235..1783045	106810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T24186 WP_000813254.1 NC_013008:1747746-1747901 [Escherichia coli O157:H7 str. TW14359]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T24186 NC_013008:1747746-1747901 [Escherichia coli O157:H7 str. TW14359]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT24186 NC_013008:c1747734-1747676 [Escherichia coli O157:H7 str. TW14359]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin