Detailed information of TA system TA241854

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3101864..3102662
• Replicon: chromosome
• Accession: NZ_CP095271
• Organism: Escherichia coli strain EC9682

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: MU524_RS15490
• Protein ID: WP_001094434.1
• Coordinates: 3101864..3102238 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MU524_RS15495
• Protein ID: WP_001552747.1
• Coordinates: 3102285..3102662 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU524_RS15465 (3097645)	3097645..3098481	-	837	WP_247164748.1	DUF4942 domain-containing protein	-
MU524_RS15470 (3098662)	3098662..3100203	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
MU524_RS15475 (3100218)	3100218..3100964	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
MU524_RS15480 (3101167)	3101167..3101364	-	198	WP_000839257.1	DUF957 domain-containing protein	-
MU524_RS15485 (3101376)	3101376..3101867	-	492	WP_000976859.1	DUF5983 family protein	-
MU524_RS15490 (3101864)	3101864..3102238	-	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
MU524_RS15495 (3102285)	3102285..3102662	-	378	WP_001552747.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
MU524_RS15500 (3102712)	3102712..3103356	-	645	WP_000086759.1	hypothetical protein	-
MU524_RS15505 (3103375)	3103375..3103596	-	222	WP_000692315.1	DUF987 domain-containing protein	-
MU524_RS15510 (3103659)	3103659..3104135	-	477	WP_001186727.1	RadC family protein	-
MU524_RS15515 (3104151)	3104151..3104624	-	474	WP_001542276.1	antirestriction protein	-
MU524_RS15520 (3104718)	3104718..3104963	-	246	WP_001164966.1	hypothetical protein	-
MU524_RS15525 (3104963)	3104963..3105784	-	822	WP_001234585.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T241854 WP_001094434.1 NZ_CP095271:c3102238-3101864 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T241854 NZ_OU594967:367253-368140 [Celerinatantimonas diazotrophica]
ATGCTGAAGCAACAAATTCGCCAAATCGTACAGTCAAATCCTGACTATGCAGCAGTAACCCCCGTGATAGAAAAAGAGAT
CTTGCATCATGACATTATGGATGTGCTGATCAAACAAGGTGTTCTGCAGCGATTGACCTTTATTGGCGACACCTCATTAC
GCATGTGTTACAACAGCAGTCGGTTATCAGAGGATCTTGATTTCAACGGTGGTGATGATTTCAAACCATCCGAGTTTGAG
GGGCTGGAGTCCGAAATCCAGACCTATATTCAAAATAAGTATGAAACCGAAGTTTGGGTAAGCAAGCCCTCTGGCGAGCA
GCAAGGGAATACCGTTTCGTGGAAAATTAGCATCGTGAAAGAGCCTAATCGTCCTGATTTACCGCGGCAAAAAATGCATA
TTGATGTCTGTGCGATGCCCTCATTCGATATCGAAAAGCGTCCGTTAATCAATCATTACGACATTGTTGTCCCGACTGAA
GGCATCTTGGTCCCTGTACAGTCACTACAAGAGATATTGGCCGATAAGCTTATTGCGTTAGCGTATCGAGCAAGGCGTAT
TAAGCCTCGTGATATCTGGGATATTGTTTGGATTAAGCAGCGTGGGATTGATTTATCCAAGGTATTGGTTGAAAAGAAAC
TCAACGCCAGACACAAGCAAACAGATGACTTTAGACAAATGCTGTCTACTGCGTTAGCCAAATTAATGTTGGAAGACGAA
GTACACAATGACTTCAATATGGAAATGAGCCGTTTTATCCCCAAGCAAATTAAAGAGAGAACTTTAGATAACCCAGAGTA
TTGGACATACGTTCAGGGTGAGGTAAATGCCATCGCATCTGAGTTACTTCATGATGACACAGCCAAAAATCCATTTGATA
TGGGTTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13757.63 Da
• Isoelectric Point: 6.3127

>AT241854 WP_001552747.1 NZ_CP095271:c3102662-3102285 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT241854 NZ_OU594967:366700-367224 [Celerinatantimonas diazotrophica]
ATGGCTAAATCTGATGAGCTACTCAGCGCATTAGCAAAAGCGGTTAACGCTGGCGGTGGCGTGCACAGTGCAACTGAGCT
TGCTTTTATGTTGGGCGAGCCTTGTGACCCAGCTTTTAGAAAGTTTCTCGCTGATAGCGTAAAGAAGGGCTTACTAAGAC
GAGTGGTGAAAGGCTTCTATGAAAGTATGATTACACCACCTGAGCCTGAGACGGCTATTTATAAAATCATCAAAAAATTG
CGTAGTGGCGTATTGAACTATATCAGCCTAGAAAGTCAGCTCAGCTATACCGGCGATATTTCACAAATTATGATGGGCAG
AGTCACGGTAGTGACTAAAGGTCGGAGTGGTTGTTTTGATACGCCTTATGGCGTGATCGAATTTACCCACACTAAGAAGC
CAGTCGAACAAATCGCACTCCATTTGTACTATGACTCCGACATCAAAATGTATCGCGCAAACAGAGCACAAGCGCTTGCA
GATCTAAAGCACTGCCGACGCAATCTACATATGTTGGAGCGCTAA

Structures

Toxin

• AlphaFold DB: Q1RDD2