Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 885502..886156 Replicon chromosome
Accession NZ_CP095271
Organism Escherichia coli strain EC9682

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag MU524_RS04330 Protein ID WP_000244781.1
Coordinates 885749..886156 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MU524_RS04325 Protein ID WP_000354046.1
Coordinates 885502..885768 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU524_RS04305 (881590) 881590..883023 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
MU524_RS04310 (883068) 883068..883379 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
MU524_RS04315 (883543) 883543..884202 + 660 WP_000250274.1 hemolysin III family protein -
MU524_RS04320 (884279) 884279..885259 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
MU524_RS04325 (885502) 885502..885768 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MU524_RS04330 (885749) 885749..886156 + 408 WP_000244781.1 protein YgfX Toxin
MU524_RS04335 (886196) 886196..886717 - 522 WP_001055874.1 flavodoxin FldB -
MU524_RS04340 (886829) 886829..887725 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
MU524_RS04345 (887750) 887750..888460 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MU524_RS04350 (888466) 888466..890199 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T241833 WP_000244781.1 NZ_CP095271:885749-886156 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T241833 NZ_OU342919:c4217655-4217479 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT241833 WP_000354046.1 NZ_CP095271:885502-885768 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT241833 NZ_OU342919:c4217433-4217017 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References