Detailed information of TA system TA241772

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 111909..112335
• Replicon: plasmid p21080534_1
• Accession: NZ_CP095248
• Organism: Klebsiella pneumoniae strain 21080534

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MU311_RS27500
• Protein ID: WP_001372321.1
• Coordinates: 111909..112034 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	112111..112335 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU311_RS27465 (106964)	106964..107191	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
MU311_RS27470 (107285)	107285..107971	-	687	WP_015059009.1	PAS domain-containing protein	-
MU311_RS27475 (108162)	108162..108545	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MU311_RS27480 (108822)	108822..109469	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
MU311_RS27485 (109766)	109766..110587	-	822	WP_001234445.1	DUF932 domain-containing protein	-
MU311_RS27490 (110698)	110698..110994	-	297	WP_001272251.1	hypothetical protein	-
MU311_RS27495 (111294)	111294..111590	+	297	Protein_132	hypothetical protein	-
MU311_RS27500 (111909)	111909..112034	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MU311_RS27505 (111976)	111976..112125	-	150	Protein_134	plasmid maintenance protein Mok	-
- (112111)	112111..112335	-	225	NuclAT_0	-	Antitoxin
- (112111)	112111..112335	-	225	NuclAT_0	-	Antitoxin
- (112111)	112111..112335	-	225	NuclAT_0	-	Antitoxin
- (112111)	112111..112335	-	225	NuclAT_0	-	Antitoxin
MU311_RS27510 (112347)	112347..113066	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
MU311_RS27515 (113063)	113063..113497	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MU311_RS27520 (113566)	113566..115589	-	2024	Protein_137	ParB/RepB/Spo0J family partition protein	-
MU311_RS27525 (115650)	115650..115883	-	234	WP_000006003.1	DUF905 family protein	-
MU311_RS27530 (115941)	115941..116468	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
MU311_RS27535 (116770)	116770..117219	+	450	Protein_140	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaSHV-12	rmpA2 / iutA / iucD / iucC / iucB / iucA / iucA	1..210914	210914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T241772 WP_001372321.1 NZ_CP095248:c112034-111909 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T241772 NZ_OU015345:c2141822-2141481 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT241772 NZ_CP095248:c112335-112111 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin